GREEN SYNTHESIS OF NANO-HYDROXYAPAPTITE USING EGGSHELL WASTE Vijay Hiraman Ingole (Ph.D student) Dept. of Nanotechnology, Dr B A M U Aurangabad, Maharashtra, India Guided by: Dr. Anil V Ghule, Professor Department of Chemistry Shivaji University Kolhapur, Maharashtra, India E-mail: mvt.vijay@gmail.com Mob: 08793294125

Outline Introduction Methodology Result and Discussion Conclusion Acknowledgments Outline of presentation 2 2

Hydroxyapatite The main mineral component of human teeth and bones Introduction Hydroxyapatite The main mineral component of human teeth and bones Non-Immunogenic Non-inflammatory HAp has all characteristics features of Biomaterial Biocompatible Bioactive Osteoconductive Non-toxic HAp Synthetic hydroxyapatite [Са10(РО4)6(ОН)2 ]the best biocompatible material

Introduction Hydroxyapatite (HAp) have been explored considerably over the last many decades due to its potential Bio-medical and industrial applications. HAp is white , naturally occuring apatite also have brown, yellow , or green colorations. 50% of bone is made up of a modified form of the inorganic mineral HAp known as bone mineral. HAp could synthesis by various physico-chemical methods such as sol gel, hydrothermal, ball milling etc. Green synthesis pave the way to avoid hazardous chemical, sophisticated instruments which reduces the cost.

Introduction Moreover, recycling of waste material is widely assumed to mitigate resource scarcity, which decreases environmental pollution and minimize energy consumption. Literature survey gives the evidence for use of nano-HAp and nanostructured bio-material as artificial bone material have the ability to develop & design the properties analogous to the natural bone.

Introduction Research on Biomaterials and Nano-biomaterials for bone implantation & replacement has expanded considerably over the last four decades. This research in India is of utmost importance. With this motivation, in the present study, ultrasound assisted biosynthesis of hydroxyapatite nanoparticles (Bio-HAp) has been demonstrated using extract of Gram bean (Cicer arietinum L.).

Methodology

Characterization: X-ray diffraction (XRD), scanning electron microscope (SEM), FT-IR, Raman spectroscopy.

XRD spectra of (-) Bio-Hap, (-) Bio-HAp600 . Result and Discussion XRD spectra of (-) Bio-Hap, (-) Bio-HAp600

Result and Discussion (a) (b) (b) Scanning electron microscope (SEM) images of (a) Bio-Hap, (b) Bio-HAp600

FTIR of Bio-HAp and Bio-HAp600

Raman spectra of Bio-Hap600

Conclusion Successful synthesis of Bio-HAp from eggshells by using gram bean extract and sono-chemical method . This was confirmed by the XRD, FT-IR and Raman spectra. The morphology of the synthesized Bio-HAp crystals was examined by SEM.

Personal Introduction Vijay Hiraman Ingole Ph D Student, Department of Nanotechnology, Dr Babasaheb Ambedkar Marathwada Univ., Aurangabad Birth: July 23, 1983 Qualification:M.Sc.(Bio-Physics), M.Phil., (Bio-Tech) Teaching Experience: Assistant lecturer –Department of Physiology, Shri. Vasantrao Naik, Government Medical College. Yavatmal,Maharashtra, India. From 8/8/05 to 6/4/06 (8 months) Assistant Professor –Department of Physiology, S. B. K. S. Medical Institute & Research Centre, Sumandeep Vidyapeeth Piparia Tq. Waghodia Dist. Baroda, Gujarat, India. From 26/06/2006 to 20/10/2012 ( 06 years and 03 months) 

Acknowledgement Collaborator and Funding Agencies Special thanks to UGC-New Delhi for awarding me UGC- Rajeev Gandhi Fellowship. Dr. Babasaheb Ambedkar Marathwada University, Aurangabad Dr. Anil V. Ghule, Professor, Department of Chemistry, SU Kolhapur. Dr. Ramphal Sharma, Head, Dept. of Nanotechnology, BAMU A’bad. My colleagues Ketan , Anil, Ganesh, Swapnali and all from Thin Film Laboratory C-MET Pune http://www.bamu.net http://www.chem.nthu.edu.tw

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