Trimethylation of histone 3 at lysine 4 in cryopreserved bovine embryos produced in vivo with sexed semen Mirela B. Souza Cáceres, Wilian A. Leite da.

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Trimethylation of histone 3 at lysine 4 in cryopreserved bovine embryos produced in vivo with sexed semen Mirela B. Souza Cáceres, Wilian A. Leite da Silva, Ana C. Bini de Lima, Jair S. de Oliveira, Christopher J. Tavares Cardoso, Jonathan V. dos Santos, Evelyn R. Andrade, Mauricio M. Franco, Ralf Poehland, Fabiana de Andrade Melo Sterza Theriogenology Volume 86, Issue 8, Pages 1944-1952 (November 2016) DOI: 10.1016/j.theriogenology.2016.06.013 Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 1 Experimental design: superovulation protocol, TAI (twice on Day 9), and uterine flushing. EB, estradiol benzoate; P4, progesterone-P4 intravaginal device; PGF2α, prostaglandin F2α; TAI, time-fixed artificial insemination. Theriogenology 2016 86, 1944-1952DOI: (10.1016/j.theriogenology.2016.06.013) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 2 Identification of H3K4me3 in morulae obtained after uterine flushing 7 days after artificial insemination with conventional or sexed semen. Immunofluorescence analysis by confocal microscopy. Blue: anti-H3K4me3 antibody, green: SYBR Green, merge: anti-H3K4me3 + SYBR Green. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Theriogenology 2016 86, 1944-1952DOI: (10.1016/j.theriogenology.2016.06.013) Copyright © 2016 Elsevier Inc. Terms and Conditions

Fig. 3 Identification of H3K4me3 in blastocysts obtained after uterine flushing 7 days after artificial insemination with conventional or sexed semen. Immunofluorescence analysis by confocal microscopy. Blue: anti-H3K4me3 antibody, green: SYBR Green, merge: anti-H3K4me3 + SYBR Green. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) Theriogenology 2016 86, 1944-1952DOI: (10.1016/j.theriogenology.2016.06.013) Copyright © 2016 Elsevier Inc. Terms and Conditions