Multiparametric flow cytometry of normal human bone marrow:

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Supplementary Figures

Presentation transcript:

Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies GEIL-GTLLF 2008 Part four : Lymphocytic maturation IV.1

Backgating Display Strategy IV.2 Lymphober CD45 SSC FLn SSC CD45 SSC FSC FLx FLy Lymphober CF IV.2

Informative combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD11b/CD16 p. IV.4 CD24/CD10 p. IV.9 Tube 5 : CD38/DR/CD34/CD45 Tube 10 : CD7/CD117/CD34/CD45 CD38/CD34 p. IV.5 CD7/CD117 p. IV.10 CD7/CD34 p. IV.11 . Tube 6 : CD38/CD10/CD19/CD45 CD38/CD10 p. IV.6 Tube 11 : CD22/CD10/CD20/CD45 CD22/CD10 p. IV.12 Tube 8 : CD34/CD56/CD19/CD45 CD10/CD20 p. IV.13 CD34/CD19 p. IV.7 CD56/CD19 p. IV.8 CF Modifs j IV.3

CD11b/CD16 Lymphober Gating CD11b Gating CD16 IV.4 CD45 SSC FSC FLy FLx CD11b/CD16 CD45ECD CD11bFITC SSC-Height SSCHeight CD16PC5 CD16PC6 Lymphober Gating CD11b Gating CD16 IV.4

CD38/CD34 Lymphober Gating CD38 Gating CD34 IV.5 CD45 SSC FSC FLy FLx CD45ECD CD38FITC CD38ITC SSC-Height SSCHeight CD34PC5 Lymphober Gating CD38 Gating CD34 IV.5

CD38/CD10 Lymphober Gating CD38 Gating CD10 IV.6 CD45 SSC FSC FLy FLx CD45ECD CD38FITC SSC-Height SSCHeight CD10PE Lymphober Gating CD38 Gating CD10 IV.6

CD34/CD19 Lymphober Gating CD34 Gating CD19 IV.7 CD45 SSC FSC FLy FLx CD45ECD CD34FITC SSC-Height SSCHeight CD19PC5 CD19PC6 Lymphober Gating CD34 Gating CD19 IV.7

CD56/CD19 Lymphober Gating CD56 Gating CD19 IV.8 CD45 SSC FSC FLy FLx CD45ECD CD56FITC CD56ITC SSC-Height SSCHeight CD19PC5 CD19PC6 Lymphober Gating CD56 Gating CD19 IV.8

CD24/CD10 Lymphober Gating CD24 Gating CD10 IV.9 CD45 SSC FSC FLy FLx CD45ECD CD24PE SSC-Height SSCHeight CD10PC5 Lymphober Gating CD24 Gating CD10 IV.9

CD7/CD117 Lymphober Gating CD7 Gating CD117 IV.10 CD45 SSC FSC FLy FLx CD45ECD CD7FITC SSC-Height SSCHeight CD117PE Lymphober Gating CD7 Gating CD117 IV.10

CD7/CD34 Lymphober Gating CD7 Gating CD34 IV.11 CD45 SSC FSC FLy FLx CD45ECD CD7FITC SSC-Height SSCHeight CD34PC5 Lymphober Gating CD7 Gating CD34 IV.11

CD22/CD10 Lymphober Gating CD22 Gating CD10 IV.12 CD45 SSC FSC FLy FLx CD45ECD CD22FITC SSC-Height SSCHeight CD10PE Lymphober Gating CD22 Gating CD10 IV.12

CD10/CD20 Lymphober Gating CD20 Gating CD10 IV.13 CD45 SSC FSC FLy FLx CD45ECD CD10PE SSC-Height SSCHeight CD20PC5 Lymphober Gating CD20 Gating CD10 IV.13