Inflammatory gene expression profiles in Crohn's disease and ulcerative colitis: A comparative analysis using a reverse transcriptase multiplex ligation-dependent.

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Inflammatory gene expression profiles in Crohn's disease and ulcerative colitis: A comparative analysis using a reverse transcriptase multiplex ligation-dependent probe amplification protocol Serban Gologan, Razvan Iacob, Daniela Iancu, Speranta Iacob, Bogdan Cotruta, Roxana Vadan, Ana-Maria Catuneanu, Ileana Constantinescu, Ligia Barbarii, Cristian Gheorghe, Mircea Diculescu Journal of Crohn's and Colitis Volume 7, Issue 8, Pages 622-630 (September 2013) DOI: 10.1016/j.crohns.2012.08.015 Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions

Figure 1 Profiles of mRNAs for genes encoding proinflammatory cytokines and mediators in L and N probes from IBD patients (mean values). Journal of Crohn's and Colitis 2013 7, 622-630DOI: (10.1016/j.crohns.2012.08.015) Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions

Figure 2 Differences in cytokine expression in affected mucosa (L) from CD and UC. Higher levels of IL-4 (a) and IL-12p35 (b) in CD, and of IL-8 (c), SCYA3 (d), SCYA4 (e) and GSTP1 (f) in UC are obtained. Cytokine levels are expressed as normalized probe signals, considering PARN gene as reference. Journal of Crohn's and Colitis 2013 7, 622-630DOI: (10.1016/j.crohns.2012.08.015) Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions

Figure 3 Significant differences in cytokine expression in non-affected mucosa (N) from CD and UC. Higher levels of IL1R (a) and IL-12p35 (b) in CD, and of IL-15 (c) and SCYA8 (d) in UC are obtained. Cytokine levels are expressed as normalized probe signals, considering PARN gene as reference. Journal of Crohn's and Colitis 2013 7, 622-630DOI: (10.1016/j.crohns.2012.08.015) Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions

Figure 4 ROC curve for the diagnostic score calculated using significant molecular variables (according to the multivariate analysis — Table 2) and ASCA IgG titers, that could be used to differentiate between CD and UC phenotype (c=0.86). The score was calculated using the following equation:IBDscore=1/1+EXP−−1.66+0.05×ASCAIgG−2.85×SCYA3−2.38×SCYA4−10.36×SCYA8n−52.22×IL15n+2.34×IL1Rn+1.46×IL12p35n.In the equation the symbols denote ASCA IgG titers and the respective PARN normalized ratios provided by RT-MLPA for each cytokine. Journal of Crohn's and Colitis 2013 7, 622-630DOI: (10.1016/j.crohns.2012.08.015) Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions

Figure 5 ROC curve for ASCA-cytokine score for B2–B3 CD vs B1 CD. The score was calculated based on the following equation:SevereCDscore=1/1+EXP−−0.51+0.03×ASCAIgG−222.71×IL2+61.52×IL2n−71.11×IL10n.In the equation the symbols denote the ASCA IgG titers and the respective PARN normalized ratios provided by RT-MLPA for each cytokine. Journal of Crohn's and Colitis 2013 7, 622-630DOI: (10.1016/j.crohns.2012.08.015) Copyright © 2012 European Crohn's and Colitis Organisation Terms and Conditions