Discussion & Conclusion

Slides:



Advertisements
Similar presentations
Enhancement of auditory fear conditioning after housing in a complex environment is attenuated by prior treatment with amphetamine Lisa A. Briand, Terry.
Advertisements

Estradiol Exacerbates Anxiety During Acute Methamphetamine Withdrawal in Female Mice Anthony S. Rauhut, Munin Streitz, Alicia Trappanese, Charlotte Woody.
Bisphenol A (BPA) BENJAMIN G. MUÑIZ. Origins  First synthesized in 1891 by A. P. Dianin (Hallberg et al., 2008)  Estrogenic properties identified by.
Risk for psychiatric diseases following cannabis abuse in adolescence :an experimental study Daniela Parolaro Univ of Insubria.
Methods Results Conclusions Walking the plank: Role of the medial septum in distance estimation S.S. Winter; M.M. Martin; D.G. Wallace Dept Psychology,
Examination of Age-Related Cholinergic Activity during Nicotine Exposure and Withdrawal Luis M Carcoba, M.D, Ph.D. University of Texas at El Paso Department.
Bisphenol A Lucas Stoiber CBE 555 Oct. 7 th 2008.
Triad of systems mediate response to stress.
Adult hippocampal neurogenesis is functionally important for stress-induced social avoidance Lagace et al. Sorry James, there is no serotonin in this paper…
The effects of metabotropic glutamate receptors in NMDA receptor dependent long-term potentiation in hippocampus Zhou Su-ya.
1 Psychology 304: Brain and Behaviour Lecture 22.
Physiological Explanations for Changes in the Brain with Age.
The Effect of Sensory Deprivation on Neuroplasticity Neuroplasticity: refers to the brain’s ability to rearrange the connection between its neurons – that.
1 Psychology 304: Brain and Behaviour Lecture 21.
Introduction to Synthesis 5-minute workshop. What is synthesis? Integration of knowledge from multiple sources to develop and support an argument NOT.
FGF2 blocks PTSD symptoms via an astrocyte-based mechanism
Jiří Palát Modern Methods for Analyses of Organic Pollutants
Timing of SCH23390 Administration Influences Extinction of Conditioned Hyperactivity in Mice Anthony S. Rauhut 1,2, Kristen Ratner2, Sandy Buck2, and Ee-Rah.
Hex-Tox 논문초독회 이 장 우.
CONCLUSIONS & FUTURE DIRECTIONS:
CONCLUSION/ CLINICAL IMPLICATIONS REFERENCES/ACKNOWLEDGEMENTS
Neuroscience Drug Discovery DK, H. Lundbeck A/S, Valby, Denmark
The Impact of Smoking Cessation on Real-World Prospective Memory
in the Rat Pup Ultrasonic Vocalization Model
Learning and Memory in Female Rats:
Aging.
From: Epigenetic Manipulation of Brain-derived Neurotrophic Factor Improves Memory Deficiency Induced by Neonatal Anesthesia in Rats Anesthes. 2016;124(3):
Impact of chronic cocaine on the cholesterol metabolism in the brain.
PET Scan -Increased FDG uptake PET Scan -Decreased FDG uptake
Carl W. Cotman, Nicole C. Berchtold  Trends in Neurosciences 
Stress and Our Neurons Jess Hoynoski.
Progress Report U Aims 1 & 2: To test L-NAP and other compounds for therapeutic potential in preventing alcohol-induced damage to the cerebellum.
Examination of the State Dependent Properties of WIN on Spatial Learning and Memory in Rats in the Sand Maze   Ashley R. Smith and Gretchen Hanson.
Volume 7, Issue 3, Pages (May 2014)
Lasting Epigenetic Influence of Early-Life Adversity on the BDNF Gene
Elizabeth A. Phelps, Joseph E. LeDoux  Neuron 
APES Ch.15 Environmental Hazards
Lasting effects of Estrogen and Bisphenol-A exposure during
Methods Objectives Results Conclusions References
Neuronal plasticity and antidepressant actions
Alexander W. Johnson  Trends in Neurosciences 
Fig. 6. C3 deficiency resulted in partial sparing of neuron loss in hippocampal CA3 in 16-month-old APP/PS1 mice. C3 deficiency resulted in partial sparing.
이 장 우.
Amar Sahay, Donald A. Wilson, René Hen  Neuron 
Brain regional vulnerability to anaesthesia-induced neuroapoptosis shifts with age at exposure and extends into adulthood for some regions  M. Deng, R.D.
Volume 75, Issue 4, Pages (August 2012)
Li He, Jamie Fong, Mark von Zastrow, Jennifer L Whistler  Cell 
Density of dendritic spines on L2/3 pyramidal neurons is greater in visual cortex of germline PirB−/− mice than in PirB+/+ mice at P30. Density of dendritic.
Corticotropin-Releasing Factor in the BNST
Dexmedetomidine-mediated neuroprotection against sevoflurane-induced neurotoxicity extends to several brain regions in neonatal rats  J.F. Perez-Zoghbi,
Volume 74, Issue 3, Pages (May 2012)
Volume 74, Issue 1, Pages (April 2012)
Does moderate drinking harm the fetal brain
Volume 57, Issue 2, Pages (January 2008)
Bruce S. McEwen, John H. Morrison  Neuron 
Volume 23, Issue 1, Pages (April 2018)
Transient and Persistent Dendritic Spines in the Neocortex In Vivo
Effects of Bisphenol A on Cancer Cell Proliferation
Volume 43, Issue 1, Pages (July 2004)
Volume 16, Issue 6, Pages (August 2016)
Volume 23, Issue 2, Pages (January 2013)
Hideyuki Murakami, Katsutoshi Yayama, Lee Chao, Julie Chao 
John E. Lisman, Anthony A. Grace  Neuron 
Volume 23, Issue 11, Pages (November 2015)
SeqFISH Accurately Detects Transcripts in Single Cells and Reveals Robust Spatial Organization in the Hippocampus  Sheel Shah, Eric Lubeck, Wen Zhou,
Volume 57, Issue 2, Pages (January 2008)
Intraperitoneal injection of tBHQ reduced sensorimotor deficit and stroke damage 24 h after ischemia-reperfusion. Intraperitoneal injection of tBHQ reduced.
PTZ-induced neuronal activity visualized by IEGs
Fig. 6. C3 deficiency resulted in partial sparing of neuron loss in hippocampal CA3 in 16-month-old APP/PS1 mice. C3 deficiency resulted in partial sparing.
At P23, dendritic spine density on L2/3 pyramidal cells in PirB−/− visual cortex is similar to that of PirB+/+. At P23, dendritic spine density on L2/3.
Presentation transcript:

Discussion & Conclusion Effects of Estrogen and Bisphenol-A on Adolescent Brain Development: A Golgi Study Michael Riccio, Jennifer Hagedorn, Emma Madden (Mentor: M. Frankfurt) Department of Biology and Psychology Results CA1 Pyramidal Cells CA1 Granule Cells and Prefrontal Cortex Cells are in the process of being observed and analyzed. Introduction Bisphenol-A (BPA), a common environmental endocrine disruptor, modulates estrogenic, androgenic, and antiandrogenic effects throughout the lifespan. BPA is produced in large quantities globally in the manufacturing of polycarbonate plastics, the resin lining of cans, and is found in other products such as dental sealants and receipt paper. Potential health hazards exist because alterations in temperature and pH can cause leaching of BPA from plastics (for review, vom Saal & Hughes, 2005) and detectable levels of BPA have been reported in saliva, urine, blood, breast milk, and the placenta of humans and animals (Biedermann et al., 2010; Geens et al., 2011; Rubin, 2011). Most research investigating the effects of BPA in animal models has focused on exposure during early prenatal and neonatal periods which results in behavioral, structural and physiological alterations; however, adolescence is another important development period that is characterized by profound hormonal changes. Furthermore, while oral administration of BPA is the main pathway of exposure, parenteral (e.g., injections) avoids first-pass metabolism in the liver and may more closely mimic the everyday exposure experienced by humans.   Our laboratory has recently shown that adolescent exposure to low-dose BPA increases anxiety and impairs memory (Diaz Weinstin, et al., 2013) when tested during adolescence. The current study was designed to determine whether adolescent low-dose BPA exposure alters spine density immediately and if its effects would carry through to adulthood. Figure 1: Bottom: coronal section through the rat hippocampus showing location of the dentate gyrus (DG) and CA1 regions. Golgi impregnated granule cells (upper left) and CA1 pyramidal cells (upper right). 10x Method Eighteen female Sprague Dawley rats ovariectomized at 21 days of age. At age 6 weeks, the rats were randomly assigned to either Control: saline Bisphenol-A exposed: Each rat received a daily subcutaneous injection, 40 µg/kg bodyweight, at the nape of the neck for one week. The BPA was initially dissolved in ethanol for stock solutions and diluted with saline for the injection. Estrogen: Each rat received a daily subcutaneous injection, 50 µg/kg bodyweight, at the nape of the neck for one week. Rats were sacrificed at day 49 and day 79 and processed for Golgi Impregnation. Brains were sliced using a cryostat and mounted to slides. Spine density on apical and basal dendrites of CA1 hippocampal region (CA1) pyramidal cells and granule cells were counted. Analysis: Two way ANOVA test used for post-hoc analysis Discussion & Conclusion Short-term, low dose BPA exposure during the critical period of adolescent development markedly decreased spine density in both basal and apical dendrites in pyramidal neurons CA1 when assessed at 49d . Dendritic spine counts for granule cells at 49 and 77 and CA1 at 77 days are in progress. The CA1 data indicate that BPA effects at 49 days are greater than those seen previously in adults. These results suggest that age is a critical factor in BPA induced plasticity during adolescence. BPA exposure in combination with Estrogen to observe the possible estrogen protection mechanism against the harmful effects of BPA. Observe the effects of BPA in other anatomical locations of the brain: Hypothalamus, Prefrontal Cortex, Amygdala, CA3. Future Studies Figure 2: Pyramidal cell     Subjects OVX(n=36) 21 42 49 77 Postnatal Day (PND) Daily s.c. injections BPA (n=24) or Vehicle (n=24) Cohort 1 sacrificed/Golgi   Cohort 2 Figure 3: Section of dendrite with spines (100x under oil)