Identification of Unknown Bacteria: individual assignment

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Presentation transcript:

Identification of Unknown Bacteria: individual assignment Lab 13 Goals and Objectives: Identification of Unknown Bacteria: individual assignment Use your data from the 12 possible unknowns for comparison and your Dichotomous Key to solve for your unknown based on the results you collect. Record results for MacConkey Agar Nutrient Gelatin Decide which collection of media you need next (get approval from instructor or PA) then make new inoculations From single colonies: Option A: MSA plate & Rabbit Serum (coagulase) Option B: BAP (hemolysis) & Bacitracin Option C: SIM (H2S, Indole, Motility), Citrate, MR & Urea

Unknown Identification Day 1: Each person sign out one unknown Make streak for isolation on BHIA Put your name on original slant and return Day 2: Gram stain Catalase test Inoculate MacConkey Agar Inoculate Gelatin Deep Day 3: Based on Gram, Gelatinase, Catalase, Lactose, set up assays to solve: A. If Staph: MSA and Coagulase B. If Strep/Entero: BAP and Bacitracin C. If Gram Neg: SIM, Citrate, MR, Urea

Lab 12&13 Lab 12&13 Lab 13 Lab 13 Lab 12 Lab 13 Option A Option B All 12 Possible Unknowns Lab 12&13 Lab 12&13 Gram Positive Gram Negative Gelatinase + Gelatinase - Gelatinase - Gelatinase + Lab 13 Lab 13 Lab 12 Lab 13 Catalase + Catalase - Lactose + Lactose - Option A Option B Option C Mannitol + OR Coagulase + Mannitol - OR Coagulase -  Hemolytic OR Bacitracin R  Hemolytic OR Bacitracin S Indole + OR Citrate - Indole - OR Citrate + H2S + OR Motile H2S - OR Nonmotile Urea + OR MR + VP - Urea - OR MR - VP + Urea + Urea - Staphylococcus epidermidis Pseudomonas aeruginosa Staphylococcus aureus Enterococcus faecalis Streptococcus pyogenes Klebsiella pneumoniae Salmonella typhimurium Enterobacter cloacae Bacillus subtilis Escherichia coli Proteus vulgaris Shigella flexneri

Identification of Unknown Bacteria: individual assignment Lab 13 Goals and Objectives: Identification of Unknown Bacteria: individual assignment Use your data from the 12 possible unknowns for comparison and your Dichotomous Key to solve for your unknown based on the results you collect. Record results for MacConkey Agar Nutrient Gelatin Decide which collection of media you need next (get approval from instructor or PA) then make new inoculations From single colonies: Option A: MSA plate & Rabbit Serum (coagulase) Option B: BAP (hemolysis) & Bacitracin Option C: SIM (H2S, Indole, Motility), Citrate, MR & Urea

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