Fig. 1. Percentage of patients with AS, with RA and of normal controls responding to the in vitro stimulation with the G1 domain of the proteoglycan aggrecan.

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Fig. 1. Percentage of patients with AS, with RA and of normal controls responding to the in vitro stimulation with the G1 domain of the proteoglycan aggrecan. Response was measured either by IFNγ or TNFα production of CD4+ T cells after antigen‐specific stimulation in comparison with stimulation without antigen. For more details see Patients and methods section. Analysis was done with whole peripheral blood. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved

Fig. 2. Example of an antigen‐specific response to the G1 domain of the proteoglycan aggrecan compared with stimulation without antigen (Ag) or with the human cartilage‐derived antigens glycoprotein (gp)‐39 or collagen II in a patient with AS. After staining for T‐cell surface markers and intracellular cytokines a gate for CD4+ T cells was set. The percentage of IFNγ/CD69‐ or TNFα/CD69‐double‐positive cells of the CD4+ T‐cell subpopulation is indicated. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved

Fig. 3. Example of an antigen‐specific response to the G1 domain of the proteoglycan aggrecan compared with stimulation without antigen (Ag) or with the human cartilage‐derived antigen collagen II in a patient with RA. After staining for T‐cell surface markers and intracellular cytokines a gate for CD4+ T cells was set. The percentage of IFNγ/CD69‐ or TNFα/CD69‐positive cells of the CD4+ T‐cell subpopulation is indicated. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved

Fig. 4. Percentage of patients with AS and with RA responding to the in vitro stimulation with the G1 domain of the proteoglycan aggrecan. Response was measured either by IFNγ or TNFα production of CD4+ T cells after antigen‐specific stimulation in comparison with stimulation without antigen. For more details see Patients and methods section. Analysis was done with whole synovial fluid. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved

Fig. 5. Example of an antigen‐specific response to the G1 domain of the proteoglycan aggrecan compared with stimulation without antigen (Ag) in a patient with AS. The T‐cell response in synovial fluid (SF) is higher than peripheral blood (PB). After staining for T‐cell surface markers and intracellular cytokines a gate for CD4+ T cells was set. The percentage of IFNγ/CD69‐ or TNFα/CD69‐positive cells of the CD4+ T‐cell subpopulation is indicated. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved

Fig. 6. Example of an AS patient with an antigen‐specific response to one of the pools of peptides (pool 4), but not to pool 1, derived from the G1 domain of the proteoglycan aggrecan compared with stimulation without antigen (Ag). Out of pool 4, containing peptides 29–38, only the single peptide 35 but not peptide 37 was recognized by this patient's CD4+ T cells. After staining for T‐cell surface markers and intracellular cytokines a gate for CD4+ T cells was set. The percentage of IFNγ/CD69‐ or TNFα/CD69‐positive cells of the CD4+ T‐cell subpopulation is indicated. From: Predominant cellular immune response to the cartilage autoantigenic G1 aggrecan in ankylosing spondylitis and rheumatoid arthritis Rheumatology (Oxford). 2003;42(7):846-855. doi:10.1093/rheumatology/keg230 Rheumatology (Oxford) | Rheumatology 42 ©British Society for Rheumatology; all rights reserved