Chapter6 Ligand-gated Channels of Fast Chemical Synapses

Slides:

Advertisements

Similar presentations

Ion Channels The plasma membrane is 6-8nm thick, and consists of a mosaic of lipids and proteins. The lipid is hydrophobic, and will not allow ions through.

Advertisements

Gated Ion Channels Ahu Karademir Andrei Vasiliev.

Ligand gated ion channels Channel structure –Heteropentamer –4-transmembrane pass subunits Neurotransmitter diversity Post synaptic potentials –Excitatory.

LECTURE 9: INTEGRATION OF SYNAPTIC INPUTS (Ionotropic Receptors) REQUIRED READING: Kandel text, Chapter 12 At neuromuscular synapse, single axonal action.

Figure 8.1 Forms of short-term synaptic plasticity.

Neuromuscular Transmission synaptic transmission between a somatic motor neuron and a skeletal muscle fiber Somatic Fig

Monosynaptic reflex. Physiology G6001 Nerve and Synapse Classical elements of synaptic transmission: Neuromuscular junction Transmitter release Synaptic.

Chemical synapses: post-synaptic mechanisms. Postsynaptic Membranes and ion channels Ligand gated ion channels – a review a. Resting K + channels: responsible.

Excitatory Amino Acids. Excitatory amino acid receptors Transmitter is L-glutamate Formed by GABA-transaminase Inactivated by uptake Receptor classification.

Part Fundamentals of Physiology Part II Food, Energy, and Temperature Part III Integrating systems Part IV Movement and Muscle Part V Oxygen, Carbon dioxide,

Neurotransmitters I The Life Cycle of a Conventional NT Biosynthesis & Storage Release Receptor Action Inactivation.

Membrane Transport Chapter 11. Transcellular transport of glucose.

DAVID SANTUCCI QUANTITATIVE BIOLOGY BOOTCAMP 2009 A BRIEF HISTORY OF THE SYNAPSE.

Neuron schematic  G t = RT ln (c 2 /c 1 ) + zF  E axon myelin sheath dendrites nerve endings nt release nt receptors Cell body synapse.

Ligand-Gated Ion Channels Molecular Biophysics 28 September 2007.

Biology for Engineers: Cellular and Systems Neurophysiology Christopher Fiorillo BiS 521, Fall , Part 5: Neurotransmitters,

Neural Plasticity: Long-term Potentiation Lesson 15.

Excitable cells and their biochemistry David Taylor

Next theme: What’s going on at the postsynaptic membrane? Ligand-gated ion channels: - ACh receptors (excitatory) - glutamate receptors (excitatory) -

Lecture 7: Stochastic models of channels, synapses References: Dayan & Abbott, Sects 5.7, 5.8 Gerstner & Kistler, Sect 2.4 C Koch, Biophysics of Computation.

Neurotransmitters & Receptors. Sensory neuron Motor neuron Receptor potentialAction potential Synaptic potential Action potential.

1 Synaptic Transmission. 2 Synaptic contacts Axodendritic – axon to dendrite Axodendritic – axon to dendrite Axosomatic – axon to soma Axosomatic – axon.

Chapter 44: Neurons and Nervous Systems CHAPTER 44 Neurons and Nervous Systems.

Sci2 Lect 5 Synaptic Transmission ©Dr Bill Phillips 2002, Dept of Physiology Fast Excitatory Postsynaptic Potentials Ligand gated ion channels Presynaptic.

Graduate Seminar, NJIT June 14, 2012 Analytical & Computational Neuroscience: Lecture 2 Action Potentials and Chemical Synapses Math 430 * Math 635 * Fall.

Neurons: Cellular and Network Properties

Neurotransmitters A. Criteria 1. must mimic presynaptic effects if administered exogenously 2. must be released during activity of presynaptic neuron 3.

Membrane Protein Channels

Review Intro K2 –functions of glial cells –parts of neurons –types of neurons –electrical transmission of information stretch reflex Genes and Behavior.

Neural Mechanisms of Learning & Memory Lesson 24.

Membrane Protein Channels Potassium ions queuing up in the potassium channel Pumps: 1000 s -1 Channels: s -1.

Action potential 1. Action potentials arriving at the presynaptic terminal cause voltage- gated Ca2+ channels to open. Ca Presynaptic terminal.

Cell to cell communication in the nervous system The synapse Electrical synapse Chemical synapse Role of calcium “neurocrines” Receptors Post-synaptic.

Nens220, Lecture 6 Interneuronal communication John Huguenard.

1 At the dendrite the incoming signals arrive (incoming currents) Molekules Synapses Neurons Local Nets Areas Systems CNS At the soma current are finally.

Synaptic Transmission / Central Synapses I Tom O’Dell Department of Physiology C8-161 (NPI), x64654.

Chapter 2 Structure and functions of cells of the nervous system.

Chapter 7 Metabotropic Channels Ligand-gated channel Metabotropic channel Ligand-gated channels nAchR NMDAR, AMPA & KAR GABA A R P 2 X 5-HT 3.

Now a new topic We go on to the output zone (and the input zone):

Copyright © 2009 Allyn & Bacon How Neurons Send and Receive Signals Chapter 4 Neural Conduction and Synaptic Transmission.

Synaptic transmission in the Neuromuscular junction 1.- Anatomy and function of the neuromuscular junction 4.- Modern experiments 2.- synaptic transmission.

The Synapse and Synaptic Transmission

Chemical synapses: post-synaptic mechanisms

The Patch Clamp Method 1976 by Erwin Neher and Bert Sakmann at the Max Planck Institute in Goettingen.

14 exam questions (8 on Lectures on lecture 4)

Signal processing/integration (AP, dendrites)

Structure of a Neuron: At the dendrite the incoming

Ch 3. Superfamily of Voltage-gated channels

ION CHANNELS AS DRUG TARGETS & CONTROL OF RECEPTOR EXPRESSION

Animal Cell Chromatin.

Neurotransmitters (NT)

Animal Cell Chromatin.

Neurotransmitter receptors

Tim Green, Stephen F Heinemann, Jim F Gusella Neuron

Chapter 49 Table of Contents Section 1 Neurons and Nerve Impulses.

Chapter 16. Postsynaptic Potentials and Synaptic Integration

1. An action potential arrives at the

Neuron Physiology.

Introduction to CNS pharmacology

1. An action potential arrives at the

Cell to Cell Communication via Ion-Channel Linked Receptors

Endocannabinoid Signaling and Synaptic Function

Lecture 19 Synaptic transmission, vesicle fusion and cycling

Animal Cell Cell Membrane.

Voltage-gated ion channels Transmembrane ion channels regulated by changes in membrane potential

Nat. Rev. Endocrinol. doi: /nrendo

Week 10: Synaptic transmission – Part 1

Cell to cell communication in the nervous system

Presentation transcript:

Chapter6 Ligand-gated Channels of Fast Chemical Synapses Receptor-operated channels ①Ligand-gated channel (ionotropic) → fast chemical synapse ②Metabotropic channel → slow cell signal Ligand-gated channel Metabotropic channel

Ligand-gated channels Metabotropic channels Ligand-gated channels nAchR NMDAR, AMPA & KAR GABAAR P2X 5-HT3 Metabotropic channels GIRK ATP-sensitive K channel (Ik,ATP) CNG channel(cyclic nucleotide-gated ch.) BK channel (Ca2+ activated K+ channel) ANO1 (Ca2+ activated Cl- channel) Some TRP channels

Ligand-gated channels nAchR, GABAA-R, Glycine R, 5-HT3R Pentamer AMPA-R, NMDA-R, KA-R Tetramer P2X channels

nAchR cloning strategy (Shoshaku Numa, Kyoto U) The first of ①Patch clamped Neher& Sakmann 1973 (1996; 노벨상) ② Purified ③ Sequenced ④ Cloned ⑤ Reconstitution in lipid bilayer ⑥ Heterologous Expression nAchR cloning strategy (Shoshaku Numa, Kyoto U) Structural homology of Torpedo californica acetylcholine receptor subunits Noda et al., Nature (1983a, b, c). Electric ray; rich in nAchR Purified a-bungarotoxin-bound protein Sequencing the N-terminal peptide Synthesis of oligo nucleotide (degenerate) Fishing out mRNAs of nAchR by hybridization

nAchR: Fast transmission in neuromuscular junction End plate potential or current: inactivation

Miniature End Plate Potential (MEPP) Poison Distribution: quantal releas NMDAR & AMPAR NMDAR; Ca2+-permeable AMPAR; Ca2+-impermeable most of fast synaptic transmission: AMPAR NMDAR. ∵ Mg2+ block → voltage dependent cf. Glutamate; major excitatory NT Once depolarized → Removal of Mg2+ block → NMDA-R activation → Na+, Ca2+ influx → synaptic plasticity such as Long term potentiation (LTP)or LTD Priming effect: Molecular basis of Learning & Memory

GABAaR Sedatives Anesthetics