Evaluation of the inactivation effect of Triton X-100 on Ebola virus infectivity  Francesca Colavita, Serena Quartu, Eleonora Lalle, Licia Bordi, Daniele Lapa, Silvia Meschi, Antonella Vulcano, Antonietta Toffoletti, Eugenio Bordi, Maria Grazia Paglia, Antonino Di Caro, Giuseppe Ippolito, Maria Rosaria Capobianchi, Concetta Castilletti  Journal of Clinical Virology  Volume 86, Pages 27-30 (January 2017) DOI: 10.1016/j.jcv.2016.11.009 Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 1 (a) Inactivation of PV2 and WNV infectivity by Triton X-100. PV2 and WNV preparations were treated with 0.1% Triton X-100 at room temperature for 60min. After the incubation period, both treated and untreated samples were back-titrated by limiting dilution on Vero E6 cells. Infectivity is expressed as Log TCID50/ml. Results are expressed as mean ±SD over four independent experiments, and the statistical analysis was performed with Student’s t-test, ***p=0.0001. (b) Dose-dependent experiments on WNV inactivation after treatment with Triton X-100 for 60min. WNV preparations were treated with different concentrations of Triton X-100 for 60min at room temperature. After the incubation period, both treated and untreated samples were back-titrated by limiting dilution on Vero E6 cells. Infectivity is expressed as Log TCID50/ml. Results are expressed as mean ±SD over three independent experiments, and statistical analysis was performed with Student’s t-test, **p=0.0013, ***p=0.0003. (c) Time-course experiments on WNV inactivation after treatment with 0.1% Triton X-100. WNV preparations were treated with 0.1% Triton X-100 at room temperature for increasing time intervals (0min: virus titration started immediately after Triton X-100 addition; 30min; 60min; 180min). Both treated and untreated samples were collected at the indicated time points and back-titrated by limiting dilution on Vero E6 cells. Infectivity is expressed as Log TCID50/ml. Results are expressed as mean ±SD over three independent experiments and statistical analysis was performed with Student’s t-test, **p=0.0027, ***p≤0.001. Journal of Clinical Virology 2017 86, 27-30DOI: (10.1016/j.jcv.2016.11.009) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 2 EBOV inactivation with 0.1% Triton X-100 for 60min of incubation. EBOV preparations were treated with and without 0.1% Triton X-100 at room temperature for 60min. After the incubation period, both treated and untreated samples were back-titrated by limiting dilution on Vero E6 cells. Infectivity is expressed as Log TCID50/ml. Results are expressed as mean ±SD over four independent experiments. The statistical analysis was performed with Student’s t-test, ***p=0.0001. Journal of Clinical Virology 2017 86, 27-30DOI: (10.1016/j.jcv.2016.11.009) Copyright © 2016 Elsevier B.V. Terms and Conditions