Exhaustive TCR Deep Sequencing Reveals That CMV Reactivation Fundamentally Resets Immune Reconstitution after Transplant and Results in Significant Deficits in the Effector Memory TCR Repertoire  Yvonne Suessmuth, Divya Koura, Knut Finstermeier, Cindy Desmarais, PhD, John Horan, MD, MPH, Amelia Langston, MD, Muna Qayed, MD, H. Jean Khoury, MD, Benjamin K. Watkins, MD, Harlan Robins, PhD, Rithun Mukherjee, Bruce R. Blazar, MD, Edmund K. Waller, MD, PhD, Aneesh Mehta, Leslie S. Kean, MD, PhD  Biology of Blood and Marrow Transplantation  Volume 21, Issue 2, Pages S69-S70 (February 2015) DOI: 10.1016/j.bbmt.2014.11.074 Copyright © 2015 Terms and Conditions

Figure 1A Longitudinal analysis of total CDB+ naive and effector memory (TEM) subsets depicted as % frequency of CDB+ T cells. Data are mean ± SEM in + CMV patients (n=7) - CMV patients (n=10) and healthy controls of (n=10). Also shown is the mean day of CMV reactivation (± SEM), depicted as purple bar. Figure 1B: Respresentative TCR deep sequencing landscapes of CDB+ Naive (left column)and CDB+ TEM (right column), showing frequencies of V and J gene combinations detected through deep sequencing from one patient without CMV reactivation (blue) and one patient with CMV reactivation (red). This representative data illustrates the increased TCR elenaity of the CDB Tem measured in the patients who reactivated CMV. Biology of Blood and Marrow Transplantation 2015 21, S69-S70DOI: (10.1016/j.bbmt.2014.11.074) Copyright © 2015 Terms and Conditions