Volume 73, Issue 6, Pages (December 2016)

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Volume 73, Issue 6, Pages 588-597 (December 2016) First characterization of the CD4 and CD8 T-cell responses to QuantiFERON-TB Plus Elisa Petruccioli, Teresa Chiacchio, Ilaria Pepponi, Valentina Vanini, Rocco Urso, Gilda Cuzzi, Lucia Barcellini, Daniela Maria Cirillo, Fabrizio Palmieri, Giuseppe Ippolito, Delia Goletti Journal of Infection Volume 73, Issue 6, Pages 588-597 (December 2016) DOI: 10.1016/j.jinf.2016.09.008 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Representative dot plots of the TB2-induced T-cell response of an active TB patient. PBMC were stimulated overnight with TB1 and TB2 antigens and analyzed by flow cytometry for the intracellular production of IFN-γ. The frequency of Mtb-specific T cells was calculated from the proportions of CD4 IFN-γ T cells and CD8 IFN-γ T cells. Journal of Infection 2016 73, 588-597DOI: (10.1016/j.jinf.2016.09.008) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Evaluation of IFN-γ production using QFT-Plus kit. The IFN-γ T-cell response was evaluated in active TB patients and in LTBI subjects with recent and remote infection, one day after whole blood incubation in TB1 and TB2 tubes of the QFT-Plus kit. Horizontal lines indicate the median. The dotted line represents the cut-off value of 0.35 IU/ml. TB: tuberculosis, LTBI: latent tuberculosis infection; IU: international unit; IFN: interferon; QFT: QuantiFERON. Journal of Infection 2016 73, 588-597DOI: (10.1016/j.jinf.2016.09.008) Copyright © 2017 The Authors Terms and Conditions

Figure 3 TB2 stimulation induces a CD8 T-cell response in active TB patients. Flow cytometric evaluation of CD4 (A) and CD8 (B) T-cell to TB1 and TB2 antigens in active TB patients and LTBI subjects with recent and remote infection. The response was scored positive if the frequency of IFN-γ producing T cells was at least 0.03%. The horizontal lines represent the medians. Statistical analysis was performed using the Mann–Whitney U test and the p value was considered significant if ≤0.05. TB: tuberculosis, LTBI: latent tuberculosis infection. Journal of Infection 2016 73, 588-597DOI: (10.1016/j.jinf.2016.09.008) Copyright © 2017 The Authors Terms and Conditions

Figure 4 Impact of TB disease severity on CD4 and CD8 T-cell responses. Flow cytometric evaluation of CD4 and CD8 responses to TB1 and TB2 antigens in active TB patients. Patients were stratified according the grade of TB severity: low and intermediate/high severity. The bars represent the number of patients responding to TB1 and TB2 stimulation. TB: tuberculosis. Journal of Infection 2016 73, 588-597DOI: (10.1016/j.jinf.2016.09.008) Copyright © 2017 The Authors Terms and Conditions