MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878.

Slides:

Advertisements

Similar presentations

Date of download: 5/27/2016 Copyright © The American College of Cardiology. All rights reserved. From: Multicentric inflammation in epicardial coronary.

Advertisements

Fig. 4. Effects of lobeglitazone (Lobe) on the transforming growth factor β (TGF-β)/Smad3 signaling pathway in kidney cell lines. (A) Effects of Lobe on.

TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma Cell Physiol Biochem 2015;35:

Cell Physiol Biochem 2014;33: DOI: /

Suppl. Fig x106 Culture of Irradiated A-CFSE

Characterization of Serum MicroRNAs Profile of PCOS and Identification of Novel Non-Invasive Biomarkers Cell Physiol Biochem 2014;33: DOI: /

Enhanced Reactive Oxygen Species Production, Acidic Cytosolic pH and Upregulated Na+/H+ Exchanger (NHE) in Dicer Deficient CD4+

Cell Physiol Biochem 2013;32: DOI: /

OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort.

Cell Physiol Biochem 2013;32: DOI: /

Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-kB Signaling Pathway Cell Physiol Biochem 2017;43:481–491 - DOI: /

Effects of Glycyrrhizin in a Mouse Model of Lung Adenocarcinoma

Cell Physiol Biochem 2015;37: DOI: /

IL-4 Modulates Macrophage Polarization in Ankylosing Spondylitis

Cell Physiol Biochem 2015;37: DOI: /

Cell Physiol Biochem 2016;38: DOI: /

Up-Regulation of TGF-β Promotes Tendon-to-Bone Healing after Anterior Cruciate Ligament Reconstruction using Bone Marrow-Derived Mesenchymal Stem Cells.

Cantharidin Inhibits the Growth of Triple-Negative Breast Cancer Cells by Suppressing Autophagy and Inducing Apoptosis in Vitro and in.

Downregulation of MicroRNA-145 Caused by Hepatitis B Virus X Protein Promotes Expression of CUL5 and Contributes to Pathogenesis of Hepatitis B Virus-Associated.

Bufalin Inhibits the Differentiation and Proliferation of Cancer Stem Cells Derived from Primary Osteosarcoma Cells through Mir-148a Cell Physiol Biochem.

Cell Physiol Biochem 2017;41:921– DOI: /

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

Cell Physiol Biochem 2013;31: DOI: /

Plasma Cell Leukemia Presenting as a Chest Wall Mass: A Case Report

FREQUENCIES AND PHENOTYPIC CHANGES OF DENDRITIC CELLS DURING ACUTE DENGUE VIRAL INFECTION Lertjuthaporn S1, Khowawisetsut L2, Keawvichit R1, Thitilertdecha.

T-Cell Lymphoma in a Patient with Neurofibromatosis Type 1 and AIDS

Nm23-H1 Regulates Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells via Arf6-Rac1 Signaling Axis Cell Physiol Biochem 2013;32: DOI: /

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small.

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells Cell Physiol Biochem 2017;41:1519–1531.

Administration of Curcumin Protects Kidney Tubules Against Renal Ischemia-Reperfusion Injury (RIRI) by Modulating Nitric Oxide (NO) Signaling Pathway Cell.

Cell Physiol Biochem 2017;44:1867– DOI: /

Epigallocatechin-3-Gallate Inhibits Matrix Metalloproteinase-9 and Monocyte Chemotactic Protein-1 Expression Through the 67-κDa Laminin Receptor and the.

The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-γ upon Exposure to Plasmodium falciparum-Infected.

Cell Physiol Biochem 2015;36: DOI: /

Cell Physiol Biochem 2016;38: DOI: /

The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene.

Volume 136, Issue 4, Pages e3 (April 2009)

Chronic Hepatitis B Infection is Associated with Decreased Risk of Preeclampsia: A Meta-Analysis of Observational Studies Cell Physiol Biochem 2016;38:

T-cell regulation during viral and nonviral asthma exacerbations

Cell Physiol Biochem 2016;39: DOI: /

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper by Haifa Hamdi, Véronique.

Expression and Function of RIG-I in Oral Keratinocytes and Fibroblasts

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D George W. Agak, Min Qin, Jennifer Nobe,

Dawn C. Newcomb, PhD, Madison G

Molecular Therapy - Nucleic Acids

Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy Meiqin Wang, MD, PhD, Ivana V. Yang,

A dynamic relationship between mucosal T helper type 17 and regulatory T-cell populations in nasopharynx evolves with age and associates with the clearance.

MicroRNA Expression Profiling Identifies miR-31 and miR-485-3p as Regulators in the Pathogenesis of Discoid Cutaneous Lupus Cristina Solé, Sandra Domingo,

Volume 85, Issue 3, Pages (March 2014)

Volume 140, Issue 5, Pages e3 (May 2011)

Serum neopterin in acute coronary syndromes

Physical exercise modulates the homeostasis of human regulatory T cells Max Weinhold, MA, Alexander Shimabukuro-Vornhagen, MD, Axel Franke, MD, Sebastian.

TSLP Directly Interacts with Skin-Homing Th2 Cells Highly Expressing its Receptor to Enhance IL-4 Production in Atopic Dermatitis Kazuki Tatsuno, Toshiharu.

Katherine G. MacDonald, BSc, Nicholas A. J

Quantity and Quality Reconstitution of NKG2A+ Natural Killer Cells Are Associated with Graft-versus-Host Disease after Allogeneic Hematopoietic Cell Transplantation

Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome Yongguang Lu, Lang Li, Hua Yan, Qiang.

Flow cytometric characterization of γδ T cells in our patient demonstrates specific expansion of a homogeneous Vδ2+ T cell population. Flow cytometric.

The Expression of Th17-Associated Cytokines in Human Acute Graft-versus-Host Disease Yuejun Liu, Yifeng Cai, Lan Dai, Guanghua Chen, Xiao Ma, Ying Wang,

CD127low/- and FoxP3+ Expression Levels Characterize Different Regulatory T-Cell Populations in Human Peripheral Blood Sandra Klein, Cosima C. Kretz,

Volume 34, Issue 3, Pages (March 2011)

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer Cell Physiol Biochem 2017;44:2455– DOI: /

Digital Medicine: A Primer on Measurement

Molecular Therapy - Nucleic Acids

Machteld M. Tiemessen, Tracey J. Mitchell, Lisa Hendry, Sean J

Volume 31, Issue 6, Pages (December 2009)

A Role for Regulatory T Cells in Cutaneous T-Cell Lymphoma; Induction of a CD4+CD25+Foxp3+ T-Cell Phenotype Associated with HTLV-1 Infection Patrick.

Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis

Increased CCL5 expression, infiltration of Treg cells, and apoptosis of CD8+ T cells in human colorectal tissues. Increased CCL5 expression, infiltration.

IL35 regulation of tumor growth is accompanied by suppression of CD4+ effector T-cell activity and expansion of Tregs. IL35 regulation of tumor growth.

Presentation transcript:

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 1. Flow cytometric analysis of circulating Treg cell frequencies in patients with CHD and CPS. PBM-Cs isolated from patients were labeled with CD4-FITC, CD25-APC and foxp3-PE antibodies. Cells were gated for CD4+T cells, after which the cells were gated for CD4+CD25+T cells and CD4+CD25+foxp3+T cells from samples obtained from AMI, UA, SA and CPS patients. The percentages of CD4+CD25+T cells and CD4+CD25+foxp3+T cell subsets in each group are shown in the panels. CHD: coronary heart disease (includes AMI, UA and SA); AMI: acute myocardial infarction; UA: unstable angina; SA: stable angina; CPS: chest pain syndrome; Treg cells: CD4+CD25+foxp3+T cells. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 2. Expression levels of miR-21, foxp3, TGF-β1 and smad7 in PBMCs and the levels of TGF-β1 in serum samples from patients with CHD and CPS. Levels of miR-21 (A), foxp3 (B), TGF-β1 (C) and smad7 (D) in PBMCs were analyzed by qRT-PCR, and serum TGF-β1 levels (E) were measured by ELISA. *P<0.05 vs. CPS; #P<0.01 vs. SA. CHD: coronary heart disease (includes AMI, UA and SA); AMI: acute myocardial infarction; UA: unstable angina; SA: stable angina; CPS: chest pain syndrome. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 3. Up-regulation of miR-21 results in decreased Treg cell frequency. PBMCs were transfected with miR-21 NS-m or miR-21-m. Cells were collected and then analyzed by flow cytometry. (A) and (C) show no significant differences between the frequencies of CD4+CD25+ T lymphocytes among the three groups; (B) and (D) show that the Treg cell frequency in the group transfected with miR-21 mimic significantly decreases compared with those of the other two groups. Treg cells: CD4+CD25+Foxp3+T cells. *p<0.01 vs. NS-m and blank. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 4. Down-regulation of miR-21 results in increased Treg cell frequency. PBMCs were transfected with miR-21-NS-i or miR-21-i. Cells were collected and then analyzed by flow cytometry. (A) and (C) show no significant differences between the frequencies of CD4+CD25+ T lymphocytes among the three groups; (B) and (D) show that the Treg cell frequency in cells transfected with miR-21-i increased compared with that of the other two groups. Treg cells: CD4+CD25+foxp3+T cells. *p<0.05 vs. NS-i and blank. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 5. Up-regulation of miR-21 results in decreased expression levels of foxp3, TGF-β1 and smad7 in PBMCs and decreased serum TGF-β1 levels in the cell culture supernatants. Cells transfected with miR-21 NS-m or miR-21-m and blank were collected and subjected to qRT-PCR analysis to examine the expression levels of miR-21 (A), foxp3 (B), TGF-β1 (C) and smad7 (D). We also used cell culture supernatants to measure the level of TGF-β1 (E) by ELISA. *P<0.05 vs. NS-m and blank. © 2015 S. Karger AG, Basel - CC BY-NC 3.0

MicroRNA-21 Negatively Regulates Treg Cells Through a TGF-β1/Smad-Independent Pathway in Patients with Coronary Heart Disease Cell Physiol Biochem 2015;37:866-878 - DOI:10.1159/000430214 Fig. 6. Down-regulation of miR-21 increases the expression levels of foxp3, TGF-β1 and smad7 in PBMCs and increases the level of TGF-β1 in the cell culture supernatants. Cells transfected with miR-21-NS-i or miR-21-i and blank were collected for qRT-PCR analysis and examined to determine the expression levels of miR-21 (A), foxp3(B), TGF-β1 (C) and smad7 (D). We also used cell culture supernatants to measure the level of TGF-β1 (E) by ELISA. *P<0.05 vs.NS-i and blank. © 2015 S. Karger AG, Basel - CC BY-NC 3.0