Quantitative and molecular analysis of noroviruses RNA in blood from children hospitalized for acute gastroenteritis in Belém, Brazil Tulio Machado Fumian,

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Quantitative and molecular analysis of noroviruses RNA in blood from children hospitalized for acute gastroenteritis in Belém, Brazil Tulio Machado Fumian, Maria Cleonice A. Justino, Joana D‘Arc Pereira Mascarenhas, Tammy K.A. Reymão, Erika Abreu, Luana Soares, Alexandre C. Linhares, Yvone Benchimol Gabbay Journal of Clinical Virology Volume 58, Issue 1, Pages 31-35 (September 2013) DOI: 10.1016/j.jcv.2013.06.043 Copyright © 2013 Elsevier B.V. Terms and Conditions

Fig. 1 Noroviruses (NoVs) GII viral load among the positive stool samples with and without NoVs RNA in blood (p=0.0472), and serum samples, represented by qRT-PCR cycle number at which the fluorescence meet the threshold in the amplification plot (Ct). Children with double infection of NoVs and group A rotavirus (n=6) are not shown. Journal of Clinical Virology 2013 58, 31-35DOI: (10.1016/j.jcv.2013.06.043) Copyright © 2013 Elsevier B.V. Terms and Conditions

Fig. 2 Phylogenetic dendrogram based on partial capsid nucleotide sequences of noroviruses (NoVs) GII strains detected from stool (n=14) and serum samples (n=2) of hospitalized children with acute gastroenteritis. NoV prototypes were obtained from GenBank, and genotypes and their respective accession numbers are indicated at the right. Fecal (F) and serum (S) samples are marked with a filled and an unfilled diamond, respectively. The scale bar at the bottom of the tree indicates distance. Bootstrap values (2000 replicates) are shown at the branch nodes and values lower than 60% are not shown. Journal of Clinical Virology 2013 58, 31-35DOI: (10.1016/j.jcv.2013.06.043) Copyright © 2013 Elsevier B.V. Terms and Conditions