Microscope Magnification.

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Presentation transcript:

Microscope Magnification

Image orientation

Depth of focus

Ocular lens (eyepiece) is 10X 4x objective lens 10x objective lens 40x objective lens 100x objective lens To get total magnification you multiply the two together: (10X) (4X) = 40X magnification (10x) (10X) = 100X magnification (10X) (40X) = 400X magnification (10X) (100X) = 1000X magnification

Elodea Plant Leaf on slide 40X 100X 400X

Cheek Cell 40X 100X 400X

How much bigger are the items viewed under a microscope? An average plant cell is 30 micrometers wide. How big is that under: 4X objective? 10X objective? 40X objective? 100X objective?

Metric Conversions

Metric conversions Because items are magnified so greatly under a microscope, it may not be appropriate to use the same units to discuss the size of something viewed with the naked eye versus the size it appears under a microscope. Using the example from the previous slide, an average plant cell is 30 micrometers in diameter. What does the diameter appear to be under a microscope using the 10X objective? State your answer in centimeters.

Practice Question The lenses shown in the photo are known as what? A. projection lenses B. eyepiece lenses C. objective lenses D. power lenses

Practice Question All the lenses shown in the photo are part of a structure of the microscope known as the what? A. rotating lenspiece B. stage C. revolving nosepiece D. arm

Practice Question How is the total magnification of the microscope is obtained? A. multiplying the power of the objective lenses together. B. multiplying the power of the objective lens by the power of the eyepiece lens. C. dividing the power of the objective lens by the power of the eyepiece lens. D. dividing the power high power objective lens by the power of the low power objective lens.

Practice Question What is best used as an adjustment on a microscope to bring a specimen into focus at high power? A. Fine focus knob B. Objective lens C. Coarse focus knob D. Eyepiece