Arterioscler Thromb Vasc Biol Effect of Macrophage-Derived Apolipoprotein E on Established Atherosclerosis in Apolipoprotein E–Deficient Mice by Weibin Shi, Xuping Wang, Nicholas J. Wang, William H. McBride, and Aldons J. Lusis Arterioscler Thromb Vasc Biol Volume 20(10):2261-2266 October 1, 2000 Copyright © American Heart Association, Inc. All rights reserved.

A, Western blot analysis of plasma apoE in apoE−/− mice 2 weeks after BMT. One microliter of undiluted plasma was electrophoresed on 12% SDS gels, transferred to nitrocellulose membranes, and probed with a polyclonal antibody to mouse apoE. A, Western blot analysis of plasma apoE in apoE−/− mice 2 weeks after BMT. One microliter of undiluted plasma was electrophoresed on 12% SDS gels, transferred to nitrocellulose membranes, and probed with a polyclonal antibody to mouse apoE. Lanes 1 through 6, mice transplanted with wild-type bone marrow; lanes 7 through 9, mice transplanted with apoE−/− bone marrow; lane 10, positive control (plasma from wild-type mice). B, PCR of DNA extracted from the peripheral blood of female apoE−/− mice 2 weeks after transplantation to amplify a 250-bp sequence of the Y chromosome. Lane 1, 100-bp ladder; lanes 2 through 8, samples from transplanted mice; lane 9, negative control (female mouse DNA); lane 10, positive control (male mouse DNA). Weibin Shi et al. Arterioscler Thromb Vasc Biol. 2000;20:2261-2266 Copyright © American Heart Association, Inc. All rights reserved.

Effects of BMT on plasma total cholesterol (A), HDL cholesterol (B), and triglyceride (C) levels in apoE−/− recipient mice. Effects of BMT on plasma total cholesterol (A), HDL cholesterol (B), and triglyceride (C) levels in apoE−/− recipient mice. Plasma lipid levels were measured before and 2, 4, 12, and 20 weeks after transplantation. ApoE−/− mice were transplanted with bone marrow from either apoE+/+ or apoE−/− mice. Values are mean±SE of 7 to 12 mice. *P<0.05 vs apoE−/−→apoE−/−. Weibin Shi et al. Arterioscler Thromb Vasc Biol. 2000;20:2261-2266 Copyright © American Heart Association, Inc. All rights reserved.

Atherosclerotic lesion areas in cross sections of aortic root from apoE−/− mice transplanted with apoE+/+ or apoE−/− bone marrow. Atherosclerotic lesion areas in cross sections of aortic root from apoE−/− mice transplanted with apoE+/+ or apoE−/− bone marrow. Each point represents a mean lesion area per section from 1 mouse. Mice were fed a chow diet during the experiment and were killed at 4, 12, and 20 weeks after transplantation. ApoE−/−→−/− represents mice transplanted with apoE−/− bone marrow and killed 20 weeks after transplantation. Weibin Shi et al. Arterioscler Thromb Vasc Biol. 2000;20:2261-2266 Copyright © American Heart Association, Inc. All rights reserved.

Representative light photomicrographs of aortic atherosclerotic lesions in apoE−/− mice transplanted with apoE+/+ or apoE−/− bone marrow. Representative light photomicrographs of aortic atherosclerotic lesions in apoE−/− mice transplanted with apoE+/+ or apoE−/− bone marrow. Sections were stained with oil red O and hematoxylin (A, B, C), an anti-mouse macrophage antibody MOMA-2 (D, E, F), or an FITC-labeled antibody to smooth muscle cell actin (G, H, I). The first and second columns represent lesions from mice transplanted with apoE+/+ bone marrow and killed at 4 and 20 weeks, respectively, after transplantation. The third column represents lesions from mice transplanted with apoE−/− bone marrow and killed at 20 weeks after transplantation. Original magnification, ×25. Weibin Shi et al. Arterioscler Thromb Vasc Biol. 2000;20:2261-2266 Copyright © American Heart Association, Inc. All rights reserved.

Immunohistochemical analysis of apoE expression in aortic atherosclerotic lesions of apoE−/− mice transplanted with apoE−/− (A) or wild-type (B) bone marrow. Immunohistochemical analysis of apoE expression in aortic atherosclerotic lesions of apoE−/− mice transplanted with apoE−/− (A) or wild-type (B) bone marrow. Sections were stained with a polyclonal rabbit anti-mouse apo E antibody. Original magnification, ×40. Weibin Shi et al. Arterioscler Thromb Vasc Biol. 2000;20:2261-2266 Copyright © American Heart Association, Inc. All rights reserved.