Evolution of Kryptolebias marmoratus from the order Teleost Using Vasa as a molecular marker by Jacob L. Perry

Background Kryptolebias marmoratus Relationship to - Oryzias latipes 1958, 1960-61- Harrington Special feature observed Specialized gonad Temperature dependent Special note: no known wild-type female Used in research - recently named model organism within last 10 years

Introduction Data at the present - limited in certain areas Vasa - DEAD-box domain, ATP-dependent-RNA helicase Helps in the development of PGC Cytoplasmic zone during embryogenesis Highly conserved region

RNA extraction, cDNA development w/ specific primers F2/R1 Materials and Methods RNA extraction, cDNA development w/ specific primers F2/R1 Fragment in FASTA format NCBI- BLAST Seaview-protein alignment RAxML7.0.4 Figtree2.1

Figure 1. Partial alignment of Vasa DEAD-box domain

Figure 2. Phylogeney of Kryptolebias marmoratus Vasa with other Figure 2. Phylogeney of Kryptolebias marmoratus Vasa with other Teleost.

Figure 3. Graphical map of Oryzias latipes Vasa gene.

Figure 4. Diagnostic gel of PCR samples, Vasa

Figure 5. Structure of DEAD-box domain from Drosophilia melanogaster. Courtesy of NCBI – PDB-ID: 2DB3

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