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Table 1 The role of microRNAs in cancers microRNAs Tumorigenesis miR-9 Neuroblastoma miR-10b Breast cancer miR-15, miR-15a Leukemia, pituitary adenoma miR-16, miR-16-1 Leukemia, pituitary adenoma miR-17-5p, miR-17-92 Lung cancer, lymphoma miR-20a Lymphoma, lung cancer miR-21 Breast cancer, cholangiocarcinoma, head & neck cancer, leukemia, cervical cancer, Pancreatic cancer miR-29, miR-29b Leukemia, cholangiocarcinoma miR-31 Colorectal cancer miR-34a Pancreatic cancer, Neuroblastoma miR-96 Colorectal cancer miR-98 Head & neck cancer miR-103 Pancreatic cancer miR-107 Leukemia, pancreatic cancer miR-125a, miR-125b Neuroblastoma, breast cancer miR-128 Glioblastoma miR-133b Colorectal cancer miR-135b Colorectal cancer miR-143 Colon cancer, cervical cancer miR-145 Breast cancer, colorectal cancer miR-146 Thyroid carcinoma miR-155 Breast cancer, leukemia, pancreatic cancer miR-181, miR-181a, miR-181c Leukemia, glioblastoma, thyroid carcinoma miR-183 Colorectal cancer miR-184 Neuroblastoma miR-196a-2 Pancreatic cancer miR-221 Glioblastoma, thyroid carcinoma, Pancreatic cancer miR-222 Thyroid carcinoma miR-223 Leukemia miR-301 Pancreatic cancer miR-376 Pancreatic cancer let-7, let-7a, let-7a-1, let-7a-3 Lung cancer, colon cancer Mol Cancer. 2007; 6: 60. Published online 2007 September 25. doi: 10.1186/1476-4598-6-60. Copyright © 2007 Cho; licensee BioMed Central Ltd.

Cell 2013 154, 311-324DOI: (10.1016/j.cell.2013.06.026)

Steven M. Johnson , Helge Grosshans , Jaclyn Shingara , Mike Byrom , Rich Jarvis , Angie Cheng , Emmanuel Labouri... RAS Is Regulated by the let-7 MicroRNA Family Cell, Volume 120, Issue 5, 2005, 635 - 647 http://dx.doi.org/10.1016/j.cell.2005.01.014

Steven M. Johnson , Helge Grosshans , Jaclyn Shingara , Mike Byrom , Rich Jarvis , Angie Cheng , Emmanuel Labouri... RAS Is Regulated by the let-7 MicroRNA Family Cell, Volume 120, Issue 5, 2005, 635 - 647 http://dx.doi.org/10.1016/j.cell.2005.01.014

Let-7g impairs proliferation and enhances cell death. Let-7g impairs proliferation and enhances cell death. (A) LKR13-Tet-On-KRAB-TE-empty and -let-7g cells were plated (5 × 105 cells per plate). Twelve hours later, cells were placed in the presence/absence of 5 μg/ml doxycycline. Forty-eight hours later, images were taken by phase contrast microscopy. (B) Small RNA Northern blot analysis was performed against let-7g and Glutamine tRNA in LKR13-Tet-On-KRAB-TE-empty, -miR-15b, and -let-7g (see SI Text for details) cells in the presence or absence of 5 μg/ml doxycycline. Kumar M S et al. PNAS 2008;105:3903-3908 ©2008 by National Academy of Sciences

Let-7g-induced tumors maintain overexpression and target suppression. Let-7g-induced tumors maintain overexpression and target suppression. (A) Small RNA Northern blotting was performed against let-7g and Glutamine tRNA in LKR13-Tet-On-KRAB-TE-empty and -let-7g tumors generated from mice treated with or without doxycycline in the drinking water as described above. (B) Western blot analysis was performed in LKR13-Tet-On-KRAB-TE-empty and -let-7g tumors generated from mice treated with doxycycline in the drinking water as described above. Kumar M S et al. PNAS 2008;105:3903-3908 ©2008 by National Academy of Sciences

K-RasG12D substantially rescues let-7g-mediated tumor suppression. K-RasG12D substantially rescues let-7g-mediated tumor suppression. (A) LKR13-Tet-On-KRAB-TE-miR-15b and -let-7g cells were infected with pBabe.Zeo.empty, K-RasG12D, and HMGA2 and Western blot analysis was performed. (B and C) LKR13-Tet-On-KRAB-TE-let-7g cells infected with pBabe.Zeo.K-RasG12D (B) or HMGA2 (C) were prepared and injected (106 cells per injection) into immune-compromised mice. Mice were treated and tumors were measured as above. Values are mean ± SEM (n = 6). Kumar M S et al. PNAS 2008;105:3903-3908 ©2008 by National Academy of Sciences

miR-26a facilitates gliomagenesis in vivo. miR-26a facilitates gliomagenesis in vivo. (A) Kaplan-Meier curves demonstrating symptom-free survival for P/Z, P/26a, P/Cre, and P/26a/Cre mice (n = 23, 19, 14, and 17 respectively; [*] P < 0.05). The percentages of tumors exhibiting WHO grade II, grade III, and grade IV histological features are also shown for each genotype. (B) Kaplan-Meier curves demonstrating symptom-free survival for P/26a mice with and without detectable immunostaining for GFP reporter (n = 11 and 8 respectively; [*] P < 0.05). Histological grades for tumors in the P/26a GFP+ and P/26a GFP− groups are also shown. Huse J T et al. Genes Dev. 2009;23:1327-1337 ©2009 by Cold Spring Harbor Laboratory Press

miR-26a represses PTEN expression in murine gliomas. miR-26a represses PTEN expression in murine gliomas. Grade IV tumors from P/Z (A–C), P/26a (D–F), and P/Cre (G–I) mice. H&E staining is shown in A, D, and G; immunostaining for PTEN is shown in B, C E, F, H, and I. C, F, and I are enlargements at 5× magnification of the regions designated in by dashed boxes in B, E, and H, respectively (bars, 400 μm). Arrows indicate tumors on H&E staining. Red and blue dots are orientation guides. (J) Quantification of PTEN immunostaining for two tumors in each genotype (see the Materials and Methods); units are arbitrary. (*) P < 0.05. Huse J T et al. Genes Dev. 2009;23:1327-1337 ©2009 by Cold Spring Harbor Laboratory Press