Modelling the cell cycle

Slides:

Advertisements

Similar presentations

Elif İŞLEK Nurse,Health Management MSc. School of Public Health Turkish Ministry of Health STRATEGIC PLANNING ON HUMAN RESOURCES FOR HEALTH IN TURKEY ( )

Advertisements

Computer modeling of radiation effects Noriyuki B. Ouchi and Kimiaki Saito Radiation Effects Analysis Research Group, Nuclear Science and Engineering Directorate,

Formulation of an algorithm to implement Lowe-Andersen thermostat in parallel molecular simulation package, LAMMPS Prathyusha K. R. and P. B. Sunil Kumar.

Ahmed Group Lecture 6 Cell and Tissue Survival Assays Lecture 6.

21th WCC, Shenzhen, China, Aug 19, 2010 Guo-Liang Jiang, MD, FACR Min Fan, MD, Jiayan Chen, MD Fudan University Shanghai Cancer Center Combination of radiation.

Tumor Growth and Radio Therapy Bettina Greese Biomathematics, University of Greifswald Nuha Jabakhanji Bioinformatics, University of Alberta.

Serial dilution and colony counting ● Serial dilution and colony counting – Also know as “viable cell counts” – Concentrated samples are diluted by serial.

Standard Treatment Not Depicted: Variation in Intensity Variation in shape.

Chapter 9 Hypothesis Testing Testing Hypothesis about µ, when the s.t of population is known.

Radiation Therapy (RT). What is cancer? Failure of the mechanisms that control growth and proliferation of the cells Uncontrolled (often rapid) growth.

Introduction to Lab Ex. 19: Enumeration of Bacteria

A Laser Afternoon: Introduction Ken Peach Particle Therapy Cancer Research Institute (Oxford Martin.

Confidence Intervals Elizabeth S. Garrett Oncology Biostatistics March 27, 2002 Clinical Trials in 20 Hours.

TREATMENT PLANNING Modelling chemo-hadron therapy Lara Barazzuol | Valencia | 19 June 2009.

First Year Workshop 2014 Miriam Lafiandra

Bacterial Growth Curve

Superiority of 160 kV vs 6 MV X- ray irradiation combined with heavy element radiosensitization By: Sara N. Lim, Anil K. Pradhan, Sultana N. Nahar, Rolf.

Effect of wind turbine mortality on noctule bats in Sweden: predictions from a simple population model Anders Hedenström & Jens Rydell Biology Department.

USE OF GEANT4 CODE FOR VALIDATION OF RADIOBIOLOGICAL PARAMETERS OBTAINED AFTER PROTON AND CARBON IRRADIATIONS OF MELANOMA CELLS Ivan Petrović 1, Giuseppe.

Slide 1 5th LHC RADIATION WORKSHOP, CERN, , Jochen Kuhnhenn, Fraunhofer INT Radiation tolerant fibres for LHC controls and communications.

Genetic and environmental conditions influencing persistence Hannes Luidalepp Institute of Technology University of Tartu August 2011.

UNIVERSAL SURVIVAL CURVE AND SINGLE FRACTION EQUIVALENT DOSE: USEFUL TOOLS IN UNDERSTANDING POTENCY OF ABLATIVE RADIOTHERAPY CLINT PARK, M.D. M.S., LECH.

E. coli evolution in low radioactivity conditions

Laboratory of Radiation Biology

Linear Energy Transfer and Relative Biological Effectiveness

Summary of t-Test for Testing a Single Population Mean (m)

Computer modelling using cellular automata of the survival fraction of cell populations under irradiation Morgiane Richard Examples.

Rosell R et al. Proc ASCO 2011;Abstract 7503.

MeV Ion Microbeams and Radiation Biology at the University of Surrey

Update Report from the Gray Cancer Institute

Chapter 9 Hypothesis Testing

CELLION Technical Report

0 Gy NSC NSC 5 Gy NSC NSC NSC NSC NSC NSC NSC NSC NSC NSC NSC NSC

Cell survival curves. A) Cells are exposed either in monolayer or in suspension culture to different doses of a drug for a given time, or to a single dose.

The representation of ice hydrometeors in ECHAM-HAM

DNA content flow cytometric histograms of propidium iodide–stained A549 cells. DNA content flow cytometric histograms of propidium iodide–stained A549.

IDH1 mutant glioma cells are sensitive to TMZ

Alternate designs for conduct and analysis of phase I cancer trials

Cellular Radiobiology

Adaptive Response to Low Dose Radiation

دانشگاه شهیدرجایی تهران

A High Content Clonogenic Survival Drug Screen Identifies MEK Inhibitors as Potent Radiation Sensitizers for KRAS Mutant Non–Small-Cell Lung Cancer Steven.

تعهدات مشتری در کنوانسیون بیع بین المللی

Radiation tolerant fibres for LHC controls and communications

BELL RINGER A student grew a yeast culture on sterilized nutrient medium in a closed dish for 5 days. Each day, she took the same size sample from the.

Clinical and Translational Radiation Oncology

The HIV protease and PI3K/Akt inhibitor nelfinavir does not improve the curative effect of fractionated irradiation in PC-3 prostate cancer in vitro and.

H-NS2-FLAG expression is upregulated in DMEM and M9 minimal medium and when cells enter the stationary growth phase. H-NS2-FLAG expression is upregulated.

Mitosis Graphic Organizer

Genetic and pharmacologic inactivation of KSR1 radiosensitizes A431 cells in vitro to the lethal effect of ionizing radiation. Genetic and pharmacologic.

A B C D Supplementary Figure S1 UMSCC1 Plateau: 24 hr Pre-Treatment

SYCE2 induces resistance to ionizing radiation and cisplatin in somatic cells. SYCE2 induces resistance to ionizing radiation and cisplatin in somatic.

A, B, apoptosis analysis using Annexin V-FITC/PI was done on 5th, 7th, and 9th day after transfection either with si control or si AEBP1in U87MG and U138MG.

PX-478 directly radiosensitizes tumor cells in vitro.

A. A. XPCKD and KIN17KD HeLa cells grow poorly 2 weeks after transfection. Forty-eight hours after transfection, cells were plated at the same density.

Anti-Flk-1 treatment inhibits growth of s. c. 4T1 and B16 tumors. s. c

Time course of a G2-M arrest in response to 6-TG compared with IR and VP-16, showing that 6-TG induced a delayed G2-M arrest in MMR+ cells. Time course.

Protocol description (randomized phase).

Irradiated whole-cell vaccination with MOC1, but not MOC2, induces immunologic memory. Irradiated whole-cell vaccination with MOC1, but not MOC2, induces.

Inhibitory effects of SFN on the TPA-induced transformation of JB6 P+ cells. Inhibitory effects of SFN on the TPA-induced transformation of JB6 P+ cells.

Growth curve (a) and doubling time (b) of glioma cells.

Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. Per1 inhibits growth and induces apoptosis in prostate cancer cell lines. LNCaP,

A protracted ketogenic diet increases radiation response in H292 lung cancer xenografts given conventional fractionated radiation. A protracted ketogenic.

WP1066 inhibits the progression of OCIM2 cells through the cell cycle.

C-MYC overexpression or growth in serum-containing media induces EMT in HBEC3p53,KRAS. c-MYC overexpression or growth in serum-containing media induces.

CDV potentiates the antitumor effect of ionizing radiation in mice intracerebrally implanted with human glioblastoma cells. CDV potentiates the antitumor.

Flavopiridol inhibits rhabdoid cell survival and induces cell cycle arrest and apoptosis. Flavopiridol inhibits rhabdoid cell survival and induces cell.

A. A. Comparison of transient HSAKIN17 gene silencing induced by either siRNA duplexes or EBV-based siRNA vectors. Twenty-four hours after seeding, HeLa.

ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared with ibrutinib. ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared.

DHA and dietary n-3 PUFAs inhibit endometrial cancer cell growth in vitro and in xenograft models. DHA and dietary n-3 PUFAs inhibit endometrial cancer.

Presentation transcript:

Modelling the cell cycle G1 phase M phase G2 phase S phase Modelling the cell cycle University of Surrey Cellion Workshop, Lund, August 2006 Morgiane Richard

Content Modelling of population growth; Modelling of the bystander effect; Experiments at the Gray Cancer Institute; Conclusions and future work

Growth of a population of cells A single cell in the centre of the dish; No radiation is applied; The time scales of glucose diffusion and cell cycle are respected: The update of the two CA are random; The probability of updating the diffusion CA is much higher than the probability of updating the biological CA (ca. 103) The simulation length represents 70 days.

Growth of a population of cells But the simulation is run for about 2 weeks! Cycling cells Cells in G1 Dead cells

Modelling the bystander effect Conditions of Schettino et al. (2005): 160 single cells on a dish; Either 1 or all cells are irradiated; Cells are let grow for 3 days and colonies are revisited. Assumptions on the bystander effect: Probability of triggering the BS: p = 4*d if dose < 0.25 and 1 otherwise. Probability to survive the BS is 0.88.

Modelling the bystander effect

Experiments at the Gray Lab Work on T98 glioma cell line; Reproduction of the HRS-like survival curve: first results promising; Work on the bystander effect induced by irradiated cell conditioned medium: No obvious bystander effect induced so far; Toxicity of irradiated medium?

Conclusion and future work Model validated for growth of population; Test of assumptions made on the bystander effect in Schettino et al. (2005); Coming soon: SF described by a linear quadratic model; BE switch on; Do we get a HRS-type survival curve?

Thank you for your attention! Any question?

Study of the bystander effect

Progress in vertical nanobeam construction

Progress in vertical nanobeam construction

Progress in vertical nanobeam construction

Progress in vertical nanobeam construction

Progress in vertical nanobeam construction w f r o m s t . 1.20 1.00 9.50 6.69 F l x b O j c W n a d k p I u ( q ) 5 6 1 3 0.50 C S g B v -