3.3 Other types of microscopy

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Presentation transcript:

3.3 Other types of microscopy Microbiology

Dark-Field Microscopy Samples are illuminated so that they appear brighter. Uses a condenser that scatters light.

Phase-contrast Microscopy Enhances contrast by splitting light.

Interference microscopy Variation of Phase-Contrast Uses a prism to split light.

Fluorescence Microscopy Specimens are chemically manipulated.

Confocal Microscopy Spatial Filtering Techniques. Eliminates out- of-focus light.

Electron Microscopy Uses magnetic coils to direct a beam of electrons from a tungsten filament.

Scanning Electron Microscope (SEM) Produces a 3- dimensional looking image of the surface of specimen.

Transmission Electron Microscope Cross-section view of a specimen

Scanned-Probe Microscopy Produces 3-D image

X-Ray Diffraction Structural Characteristics of Crystals Bragg’s Law Rosalind Franklin-DNA