Volume 15, Issue 1, Pages (January 2013)

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Volume 15, Issue 1, Pages 33-43 (January 2013) Cell therapy with bone marrow stromal cells after intracerebral hemorrhage: impact of platelet-rich plasma scaffolds Jesus Vaquero, Laura Otero, Celia Bonilla, Concepcion Aguayo, Miguel A. Rico, Alicia Rodriguez, Mercedes Zurita Cytotherapy Volume 15, Issue 1, Pages 33-43 (January 2013) DOI: 10.1016/j.jcyt.2012.10.005 Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 1 Characterization of BMSCs by flow cytometry. Rat BMSCs were labeled with CD29, CD90, CD11b, CD45 or CD31 antibodies and analyzed by flow cytometry. The rat BMSC population was identified based on forward scatter (FSC) and side scatter (SSC) properties. Of the BMSC population, ≥95% must express CD29 and CD90. Additionally, these cells must lack expression (≤5% positive) of CD11b, CD45 or CD31. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 2 (A) Rotarod test. Animals treated with BMSCs embedded in the PRP-derived scaffold started to show significant differences compared with animals treated with BMSCs in saline in the 6th month (P< 0.05). We did not see improvement throughout the study in either the saline-treated group or the PRP-derived scaffold group. (B) Locomotor activity test. Animals treated with BMSCs embedded in the PRP-derived scaffold started to show a significantly higher score (P< 0.05) than animals treated with BMSCs in saline from the 4th month. Animals treated with saline and the PRP-derived scaffold without BMSCs did not show improvement throughout the follow-up period. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 3 Macroscopic views of the brains at the end of follow-up. Brain and coronal section in an animal that received intracerebral BMSCs in saline (left). Brain and coronal section in an animal that received intracerebral administration of saline only (middle). Brain and coronal section in an animal that received BMSCs embedded in a PRP-derived scaffold (right). Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 4 Inmunohistochemical expression of Nestin antibody in the different groups. (A) BMSCs in PRP-derived scaffold, SVZ. (B) BMSCs in saline, SVZ. (C) BMSCs in PRP-derived scaffold, LZ. (D) BMSCs in saline, LZ. (E) PRP-derived scaffold only, SVZ. (F) PRP-derived scaffold only, LZ. (G) Saline only, SVZ. (H) Saline only, LZ. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 5 Inmunohistochemical expression of Doublecortin antibody in the different groups. (A) BMSCs in PRP-derived scaffold, SVZ. (B) BMSCs in saline, SVZ. (C) BMSCs in PRP-derived scaffold, LZ. (D) BMSCs in saline, LZ. (E) PRP-derived scaffold only, SVZ. (F) PRP-derived scaffold only, LZ. (G) Saline only, SVZ. (H) Saline only, LZ. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 6 Representation of the number of Ki-67-positive cells in LZ and SVZ of the different groups of the series. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions

Figure 7 Immunohistochemical findings at the level of the LZ in rats treated with BMSCs embedded in the PRP-derived scaffold. (A) Double immunostain for SrY gene (red) and NeuN (green) shows that some transplanted BMSCs can transdifferentiate to neurons and survive long-term after the transplantation procedure. (B) Double immunostain for SrY gene (red) and GFAP (green) shows that some transplanted BMSCs can transdifferentiate to astroglial cells and survive long-term after the transplantation procedure. Cytotherapy 2013 15, 33-43DOI: (10.1016/j.jcyt.2012.10.005) Copyright © 2013 International Society for Cellular Therapy Terms and Conditions