Detection of active cytomegalovirus infection in inflammatory aortic aneurysms with RNA polymerase chain reaction Shinji Tanaka, MD, Kimihiro Komori,

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Presentation transcript:

Detection of active cytomegalovirus infection in inflammatory aortic aneurysms with RNA polymerase chain reaction Shinji Tanaka, MD, Kimihiro Komori, MD, Kenichiro Okadome, MD, Keizo Sugimachi, MD, FACS, Ryoichi Mori, MD Journal of Vascular Surgery Volume 20, Issue 2, Pages 235-243 (August 1994) DOI: 10.1016/0741-5214(94)90011-6 Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Schematic design of herpesviral transcripts with RNA PCR amplification. Structures of HSV IE-1 gene (A) and cytomegalovirus IE-1 gene (B) as described in previous reports.17-19Short bold bars indicate specific probes for detection of PCR products. One hundred fifty-seven bp of HSV-1 IE-1 mRNA, 182 bp of HSV-2 IE-1 mRNA, and 230 bp of cytomegalovirus IE-1 mRNA were amplified with primer S3(1) to primer S3(2) to primer S5(2), and primer CMV19 to primer CMV22, respectively. DNA PCR primers were selected for thimidine kinase (TK) gene of HSV, and for IE-1 gene exon 4 of cytomegalovirus (primer CIE1 to primer CIE2).3 Journal of Vascular Surgery 1994 20, 235-243DOI: (10.1016/0741-5214(94)90011-6) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Histologic study of inflammatory aortic aneurysm. Lesions are characterized by thick fibrotic changes with chronic inflammation in aortic media containing lymphoid follicles. (Hematoxylin-eosin stain; original magnification × 8.) Journal of Vascular Surgery 1994 20, 235-243DOI: (10.1016/0741-5214(94)90011-6) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Detection of herpesviral DNA and RNA in human aortic tissue by PCR method. Electrophoresis of PCR products was performed on agarose gel stained with ethidium bromide. Example shows codetection of HSV with cytomegalovirus in three aortic aneurysms; two inflammatory aneurysms (IAA1, IAA2) and one atherosclerotic aneurysm (AAA1). PCR amplification was performed to detect HSV-1 DNA (HSV-D; 221 bp), HSV-1 RNA (HSV1-R; 157 bp), HSV-2 DNA (HSV2-D; 253 bp), HSV-2 RNA (HSV2-R; 182 bp), cytomegalovirus DNA (CMV-D; 230 bp), and cytomegalovirus RNA (CMV-R; 250 bp), as described in text. Positive signals were recognized in lanes IAA1/HSV1-D, IAA1/CMV-D, IAA1/CMV-R, IAA2/HSV2-D, IAA2/CMV-D, IAA2/CMV-R, AAA1/HSV2-D, and AAA1/CMV-D. Journal of Vascular Surgery 1994 20, 235-243DOI: (10.1016/0741-5214(94)90011-6) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Hypothetical alteration of aortic tissues induced by cytomegalovirus infection. Upper. Intimal cytomegalovirus infection may be one source for endothelial injury, which could promote atherosclerosis, as previously proposed by Ross.23,24Lower. Active cytomegalovirus infection in aortic media may induce synthesis of various cellular proteins, which could promote tissue fibrosis or inflammation, as hypothesized in our Discussion. CMV, Cytomegalovirus. Journal of Vascular Surgery 1994 20, 235-243DOI: (10.1016/0741-5214(94)90011-6) Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions