Invest. Ophthalmol. Vis. Sci ;57(10): doi: /iovs Figure Legend:

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From: Somatic Variants in the Human Lens Epithelium: A Preliminary Assessment Invest. Ophthalmol. Vis. Sci.. 2016;57(10):4063-4075. doi:10.1167/iovs.16-19726 Figure Legend: Work flow used to identify somatic variants in genomic DNA extracted from human lens epithelial cells. Sequencing libraries were enriched for genes of the WUCaMP2 panel (Table 2) by targeted hybridization capture. The resulting library was sequenced on an Illumina HiSeq 2500 platform to obtain paired-end reads. Sequencing results were filtered at several levels. The reads were then aligned to a human reference genome (hg19), and PCR duplicates were removed. Variants were called using SAMtools and VarScan2 software. Finally, selected variants were inspected manually using the IGV visualization tool. Date of download: 11/6/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.