Bronchoalveolar Lavage in Liquid Paraffin Pneumonitis

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Bronchoalveolar Lavage in Liquid Paraffin Pneumonitis Dominique Lauque, M.D., Georges Dongay, M.D., Thierry Levade, M.D., Claude Caratero, M.D., Pierre Carles, M.D., F.C.C.P. CHEST Volume 98, Issue 5, Pages 1149-1155 (November 1990) DOI: 10.1378/chest.98.5.1149 Copyright © 1990 The American College of Chest Physicians Terms and Conditions

Figure 1 Posterioranterior chest roentgenogram of patient 1 with liquid paraffin pneumonitis. CHEST 1990 98, 1149-1155DOI: (10.1378/chest.98.5.1149) Copyright © 1990 The American College of Chest Physicians Terms and Conditions

Figure 2 Optic micrograph of alveolar macrophages recovered by lavage from a patient with liquid paraffin pneumonitis and stained with oil Red O. The cytoplasm is full of large and small rounded vacuoles that push back the nucleus (oil red O, original magnification ×1000). CHEST 1990 98, 1149-1155DOI: (10.1378/chest.98.5.1149) Copyright © 1990 The American College of Chest Physicians Terms and Conditions

Figure 3 Electron micrograph of an alveolar macrophage recovered by lavage from a patient with liquid paraffin pneumonitis. The cytoplasm contains large, empty vacuoles, some of which are surrounded by an osmiophilic line (arrow) (original magnification × 4700). CHEST 1990 98, 1149-1155DOI: (10.1378/chest.98.5.1149) Copyright © 1990 The American College of Chest Physicians Terms and Conditions

Figure 4 Thin layer chromatography of lipids extracted from BALF of a patient with liquid paraffin pneumonitis. Left, Solvent system for phospholipid separation (chloroform/methanol/acetic acid/water: 15 + 45 +12 + 6, by vol). The plate was stained by iodine vapor. BALF (2) has an abnormal spot very slightly stained by iodine vapor and migrating as the control liquid paraffin (5). Control sphingomyelin (1), phosphatidylcholine (3), phosphatidylethanolamine (4), phosphatidylserine and phosphatidylinoditol (6), origin (o) and front (f) of migration. B is solvent system for neutral lipid separation (petroleum ether/diethyl ether/acetic acid: 80 + 20+1, by vol). BALF (MC) still has the abnormal spot migrating as control liquid paraffin (p) on the solvent front (f). Control monoglycerides (mg), diglycerides (dg), triglycerides (tg), free cholesterol (fc), cholesterol ester (ce), and fatty acid (fa). CHEST 1990 98, 1149-1155DOI: (10.1378/chest.98.5.1149) Copyright © 1990 The American College of Chest Physicians Terms and Conditions