Volume 66, Issue 1, Pages (July 2004)

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Volume 66, Issue 1, Pages 10-19 (July 2004) Lack of major involvement of human uroplakin genes in vesicoureteral reflux: Implications for disease heterogeneity Songshan Jiang, Jordan Gitlin, Fang-Ming Deng, Feng-Xia Liang, Andy Lee, Anthony Atala, Stuart B. Bauer, Garth D. Ehrlich, Sally A. Feather, Judith D. Goldberg, Judith A. Goodship, Timothy H.J. Goodship, Monika Hermanns, Fen Ze Hu, Katrin E. Jones, Sue Malcolm, Cathy Mendelsohn, Robert A. Preston, Alan B. Retik, Francis X. Schneck, Victoria Wright, Xiang Y. Ye, Adrian S. Woolf, Xue-Ru Wu, Harry Ostrer, Ellen Shapiro, Jun Yu, Tung-Tien Sun Kidney International Volume 66, Issue 1, Pages 10-19 (July 2004) DOI: 10.1111/j.1523-1755.2004.00703.x Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 1 Genomic structure of human genes encoding uroplakins (UPs) Ia, Ib, II, and III. Boxes and lines denote exons and introns, respectively. The numbers above each gene denote the detected single nucleotide polymorphisms (SNPs). Human UP Ia, UP Ib, UP II, and UP III genes span about 11.6, 18.5, 2.2,and 10.9 kb, respectively. Kidney International 2004 66, 10-19DOI: (10.1111/j.1523-1755.2004.00703.x) Copyright © 2004 International Society of Nephrology Terms and Conditions

Figure 2 Expression of uroplakin (UP) in embryonic mouse (A to E) and human (F to I) urothelium. Whole-mount (A) or paraffin sections of mouse bladder of E13 (A), E14.5 (B), E15.5 (C), and E16.5 (D), and renal pelvis of E16.5 embryos (E) were stained by immunofluorescence (A) and peroxidase (B to E). A rabbit polyclonal antiserum against total bovine UPs (A) or a mouse monoclonal antibody to UP III (B to E) was used. Note the strong UP staining of the apical urothelial surface not only in the bladder (A to D), but also in ureter and renal pelvis (E). Human fetal bladder at 70 days' gestation was paraffin-sectioned and stained using a rabbit antiserum to total bovine UPs (F and G) and a rabbit antiserum against UP Ib (H and I). (G and I) The higher magnification views of (F and H), respectively. All sections, except the whole mount in (A), were counterstained with hematoxylin. Note the strong immunostaining of the luminal surface of the fetal human bladder urothelium. Kidney International 2004 66, 10-19DOI: (10.1111/j.1523-1755.2004.00703.x) Copyright © 2004 International Society of Nephrology Terms and Conditions