Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides  K Elliott, D.S Hill, C Lambert, T.R Burroughes, P Gill  Forensic Science International  Volume 137, Issue 1, Pages 28-36 (October 2003) DOI: 10.1016/S0379-0738(03)00267-6

Fig. 1 The Arcturus PixCell II™ LM process (reproduced with permission). Forensic Science International 2003 137, 28-36DOI: (10.1016/S0379-0738(03)00267-6)

Fig. 2 (a) Profile derived from 228 sperms recovered using LM showing a mixture of male and female DNA. Despite the presence of female DNA the male profile is easily interpreted LR=4.1×107. (Arrows indicate female alleles, black circles indicate male alleles, unmarked alleles are shared). (b) A combined analysis of 228 sperms recovered using preferential lysis. The male alleles were too small to reliably score (LR=1). Forensic Science International 2003 137, 28-36DOI: (10.1016/S0379-0738(03)00267-6)

Fig. 3 (a) Profile from 79 sperms recovered 1h post-coitus (sperms isolated using LM). The balance of the profile and the lack of exclusively female alleles suggested that all the shared (unmarked) alleles present were male. Male alleles are indicated by dots (LR=71,260). (b) Profile from 128 sperms recovered 20h post-coitus (sperms isolated using LM). The larger number of female alleles present (relative to Fig. 3a) may be due in part to adherence of female DNA to the sperm (LR=1453) Female alleles are indicated by arrows. Forensic Science International 2003 137, 28-36DOI: (10.1016/S0379-0738(03)00267-6)