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© The Author(s) 2015. Published by Science and Education Publishing. Table 3. Kinetic Parameters for Manganese (II) Oxidation by Recombinant Phlebiaradiata MnP3 Mutants (E40H, E44H, E40H/E44H) in the Presence or Absence of Metal Ions [Mn2+, Zn2+, Cu2+, Co2+ and Na+ (control)]. Activity assays were performed in 100 mM Na tartrate, pH 5.0 at 25°C using varying concentrations MnSO4 and fixed concentrations 0.1 mM H2O2. Apparent kinetic parameters are estimated from the best fits to the Michaelis-Menten equation Metals Added Km (mM) kcat (s-1) kcat/Km (mM-1s-1) E40H E44H E40H/E44H MnSO4 11.0 ± 0.4 9.0 ± 0.5 20.0 ± 2.0 12.0 ± 0.2 13.0 ± 0.3 1.70 ± 0.10 1.0 1.5 0.08 0.8mM Na2SO4 13.2 ± 0.8 10.0 ± 1.0 19.0 ± 2.0 8.5± 0.2 14.0 ± 0.5 1.70± 0.07 0.6 0.09 0.8mM ZnSO4 14.0 ± 1.0 15.0 ± 1.4 17.0 ± 1.0 7.0 ± 0.2 11.0 ± 0.5 1.10 ± 0.04 0.5 0.8 0.07 0.2 mM CuSO4 47.0 ± 7.0 24.0 ± 3.0 36.0 ± 2.0 8.0 ± 0.8 5.0 ± 0.3 1.4 ± 0.2 0.2 0.04 0.8 mM CoSO4 14.3 ± 1.0 1.0 ± 0.4 17.0 ± 2.0 7.3 ± 0.2 1.20 ± 0.06 1.2 Usenobong F. Ufot et al. Role of Some Metal Ions on Steady–state Kinetics of Engineered Wild–type and Manganese (II) Binding Site Mutants of Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3). American Journal of Medical and Biological Research, 2016, Vol. 4, No. 3, 42-52. doi:10.12691/ajmbr-4-3-2 © The Author(s) 2015. Published by Science and Education Publishing.