Control of Microbial Growth

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Presentation transcript:

Control of Microbial Growth Chemical and Chemotherapeutic Agents

Chemical Control MH (one per group) Culture Quadrants Swab Filter Disk Pick one of 2 Coordinate with other table Quadrants Label for solutions (A, B, C, D) Label bacteria used Swab Confluent growth streak Rotate 90° Filter Disk Dip in proper solution Place in appropriate quad Repeat for all 4 quads A C B D

Chemotherapeutic Agents MH agar plate Group: Pairs Swab Confluent 2x (90°) AB discs dispenser seat discs

Chemical Agents MOA Isopropranol H2O2 Clorox Lab cleaner Other Lysol Betadine Chlorhexidine Denature, dissolve PM Oxidative decarboxylation Oxidation Others Disrupts plasma membranes

Minimum Inhibitory Concentration MIC

KB: measurements

Results Zones of inhibition Results Measure diameter Units: mm Table of MIC Interpretation Susceptible Intermediate Resistant

Chemotherapeutic Agents MOA Inhibit DNA gyrase Inhibit Folic acid synthesis 30S binding/interferes with mRNA Inhibit cell wall synthesis PABA analog, blocks folic acid 30S binding/interferes with tRNA 50S binding/ prevents protein elongation Naldixic acid TMPS Gentamycin Vancomycin Penicillin Sulfisoxazole Tetracycline Azithromycin

Additive and Synergistic Effects of AB Combination Therapy Reduce resistance Lower dosage Enhance activity Results Zones of inhibition Effects Additive Synergistic