RECIPE Baupte Progress meeting 08-10/11/2004

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Presentation transcript:

RECIPE Baupte Progress meeting 08-10/11/2004 UK progress Rebekka Artz Stephen Chapman Colin Campbell

I. WP01 UK Field site data Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland

Dark CO2 production 1st data point: 30/09/03

CO2 fluxes during photosynthesis

CO2 Net Ecosystem Exchange

CH4 Production exetainers exetainers exetainers exetainers

Water table 11/08/2003 13/11/2003 6/05/2004

Other parameters 1st data point: 18/12/03 1st data point: 12/02/04

Vegetation cover: Collars (0.07 m2) B C D

Vegetation cover: Grids (1 m2) B C D

II. WP2 Progress

Dark CO2 production

CO2 fluxes during photosynthesis

CH4 Production

Further work: 1st harvest expected to take place either end of november or mid march (pending decision) Clare Trinder will use the field site for litter bag experiments

III. Fungal community structure analysis by DGGE of ribosomal 18S and/or ITS sequences

CVA of fungal ITS DGGE patterns per country, grouped by site. Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

III. Fungal community structure analysis Clone library from DNA-derived ITS fragments has been prepared now cloned fragments are typed for RFLP type (in order to minimise no. of clones to be sequenced)

IV. Community level physiological profiling Method optimised for use with peat; 48 h incubation period; 14C-labeled C sources used: N-Acetyl-Glucosamine Glucose Benzoic acid Galactose Phenylethylamine Arabinose Glycine Xylose Lysine Sucrose Arginine D-Mannitol Aspartic acid Glucosamine Glutamic acid

Other data are needed to perform CCA.

CVA of CLPP patterns per country, grouped by site. Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).

V. FTIR (preliminary analysis)

CVA of FTIR patterns per country, grouped by site. Figures show Finland (A); Scotland (B); France Russey (C) and Switzerland (D).