A B C D Cytoplasmic RTCs Cytoplasmic RTCs Nuclear RTCs

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Presentation transcript:

A B C D Cytoplasmic RTCs Cytoplasmic RTCs Nuclear RTCs 35000 30000 25000 20000 Early HIV-1 DNA (copies) 15000 10000 Mouse IgG1 Rabbit IgG1 5000 IP: MA CA RT IN Vpr PML Ini1 cDNA recovery (% of cRTC DNA) 4.66 2.25 2.79 6.03 5.52 4.23 6.59 0.61 0.95 B Cytoplasmic RTCs 20000 16000 Late HIV-1 DNA (copies) 12000 8000 Mouse IgG1 Rabbit IgG1 4000 n.d. IP: MA CA RT IN Vpr PML Ini1 cDNA recovery (% of cRTC DNA) 42.16 19.98 18.46 34.87 31.77 24.56 n.d 2.69 9.42 Cytoplasmic RTCs C 9000 8000 7000 6000 5000 Early HIV-1 DNA (copies) 4000 3000 2000 Mouse IgG1 Rabbit IgG1 1000 IP: MA CA RT IN Vpr PML Ini1 cDNA recovery (% of nRTC DNA) 85.14 2.0 7.31 35.25 40.54 52.52 77.58 8.74 13.04 Nuclear RTCs D 900 5 h post-infection (100%) 800 24 h p.i. 700 600 500 Early HIV-1 DNA (%) 400 300 200 Mouse IgG1 Rabbit IgG1 100 IP: RT IN Vpr cDNA recovery (% of cRTC DNA) 1.11 0.12 3.14 22.84 1.46 3.38 0.55 1.41 Cytoplasmic RTCs