USBOT-403- Semi - IV Anther culture Dr. L. R. Rathod Dept. Of Botany M USBOT-403- Semi - IV Anther culture Dr. L. R. Rathod Dept. Of Botany M. P. A. S. C. College, Panvel. Date – 09/01/2016

Definition The culturing of pollen grains or young anthers to produce haploid plants by tissue culture is called anther culture. Additional Points: Growth of plant cells outside of an intact plant A technique essential to many areas of plant science Culture of individual or groups of cells and whole organs contribute to the understanding of both fundamental and applied science Cultures can be sustained and maintained as a mass of undifferentiated cells for an extended period of time or regenerated into whole plants

Anther/Microspore Culture

Pollen Culture Flowering plant — inflorescence — flower buds — put the flower buds in 5% teepol solution — washing in running tap water — Laminar air flow —surface sterilization — washing in S/D — culture of isolated microspore (pollen) — split the flower bud — stamen filament discard — Anther — Excised anther — Smear the anther — filter the microspore — pellet — resuspension in fresh medium — microspore —put microspore in medium — Embryogenesis — Regenerated plantlet —Regenerated haploid plant in pot.

Application of Anther or Pollen culture Utility of anther and pollen culture for basic research Use of anther and pollen culture for mutation study Use of haploid for cryogenic study Use of anther and pollen culture for plant breeding and crop improvement Application of haploid culture for horticulture plants. Anther culture and alkaloid content Haploid culture and molecular biology. Anther and pollen culture have prove very promising technique to obtain commercial varieties economically important crops like Rice, Wheat, Pepper, Coffee, Potato, grapes, mustard, pepper, coconut, and rubber plants. A single anther produces a large quantity of pollen grains and in turn each of the pollen grains is capable to produce new plant thus a very large number of plant can be produced in a short period.

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