How do we determine these structures?

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Presentation transcript:

How do we determine these structures?

Mineral Crystals

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Crystal Lattice Alexander McPherson, Introduction to Macromolecular Crystallography Wiley-Liss, 2002

Crystal Systems Jenny Pickworth Glusker, Kenneth N. Trueblood, Crystal Structure Analysis: A Primer, Oxford University Press, 1985

Light Diffraction Henry S. Lipson Crystals and X-rays Taylor & Francis 1970

X-ray Diffraction Gale Rhodes, Crystallography Made Crystal Clear: A Guide for Users of Macromolecular Models, Academic Press, 1993

X-ray Crystallography

X-ray Diffraction Photograph Rosalind Franklin’s diffraction photo of DNA

Electron Density Maps

Crystal Structures Myoglobin Hemoglobin Lysozyme Ribonuclease Myoglobin: Kendrew, Bodo, Dintzis, Parrish, Wyckoff, Phillips, Nature 181 662-666, 1958. Hemoglobin: Perutz, Proc. R. Soc. A265, 161-187,1962. Lysozyme: Blake, Koenig, Mair, North, Phillips, Sarma, Nature 206 757, 1965. Ribonuclease: Kartha, Bello, Harker, Nature 213, 862-865 1967. Wyckoff, Hardman, Allewell, Inagami, Johnson, Richards. J. Biol. Chem. 242, 3753-3757, 1967.

The Steps in the Process Isolation, Expression, Purification, Crystallization Target Selection Data Collection Structure Solution Structure Refinement Functional Annotation Publication

Vapor diffusion McPherson, A. Crystallization of Biological Macromolecules. Cold Spring Harbor Laboratory Press, 1999, p. 179.

Protein crystals McPherson, A. Crystallization of Biological Macromolecules. Cold Spring Harbor Laboratory Press, 1999, p. 185.

Crystal mounting Glusker, J. & Trueblood, K. Crystal Structure Analysis: A Primer. Oxford University Press, 1985, p. 44.

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