Fig. 1. Edited screen capture obtained during pixel counting to analyze gray matter and white matter with the ImageJ program. The region of interest area.

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Fig. 1. Edited screen capture obtained during pixel counting to analyze gray matter and white matter with the ImageJ program. The region of interest area was marked using the free drawing tool in sagittal view (left image). The "Analyze-Histogram" menu gives the pixel density value as a separate window (right image). A valley observed in the histogram represents the cut-off point between gray matter and white matter. The detailed value can be copied by clicking the "copy" button (arrow) and was then pasted into an Excel program for further analyses. Fig. 1. Edited screen capture obtained during pixel counting to analyze gray matter and white matter with the ImageJ program. The region of interest area was marked using the free drawing tool in sagittal view (left image). The "Analyze-Histogram" menu gives the pixel density value as a separate window (right image). A valley observed in the histogram represents the cut-off point. . . Anat Cell Biol. 2015 Dec;48(4):262-267. http://dx.doi.org/10.5115/acb.2015.48.4.262