Association between Mycobacterium avium subsp

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Association between Mycobacterium avium subsp Association between Mycobacterium avium subsp. paratuberculosis DNA in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls Ramón A. Juste, Natalia Elguezabal, Andrés Pavón, Joseba M. Garrido, Mariví Geijo, Iker Sevilla, José L. Cabriada, Angel Tejada, Francisco García-Campos, Roberto Casado, Itziar Ochotorena, Ander Izeta International Journal of Infectious Diseases Volume 13, Issue 2, Pages 247-254 (March 2009) DOI: 10.1016/j.ijid.2008.06.034 Copyright © 2008 International Society for Infectious Diseases Terms and Conditions

Figure 1 Representative gels of nested PCR results of MAP-specific IS900 DNA in blood samples. (A) Controls. (B) Patients. M, molecular weight marker (1 Kb ladder; New England Biolabs, Ipswich, MA, USA); lane 1, negative control of first round PCR; lane 2, negative control of second round PCR; lanes 3–17 controls (A) or IBD (B); lane 18, positive control DNA from MAP strain ATCC 19698. International Journal of Infectious Diseases 2009 13, 247-254DOI: (10.1016/j.ijid.2008.06.034) Copyright © 2008 International Society for Infectious Diseases Terms and Conditions

Figure 2 Humoral and cellular immune response. Relative increment and p-values (in parentheses) of the IBD group over the control group for mean absorbed antibody ELISA (ABMAP), IFN-γ in blood stimulated with MAP antigen (IFNMAP), or unstimulated (IFNPBS) and frequency of PCR-positive individuals. Positive values mean that the IBD group had higher means or frequencies than the controls and negative ones that they were lower. International Journal of Infectious Diseases 2009 13, 247-254DOI: (10.1016/j.ijid.2008.06.034) Copyright © 2008 International Society for Infectious Diseases Terms and Conditions

Figure 3 Proposed model for the etiology of IBD in relation to antibody and IFN-γ responses and MAP presence. IFNPBS, IFNMAP, and ABMAP values are group means. International Journal of Infectious Diseases 2009 13, 247-254DOI: (10.1016/j.ijid.2008.06.034) Copyright © 2008 International Society for Infectious Diseases Terms and Conditions