Looking at the small stuff

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Presentation transcript:

Looking at the small stuff Microscopy Looking at the small stuff

Microscopes “micro” means : “scope” means: Small “scope” means: Watch/ look It was actually developed first as entertainment for the rich

Light Microscope Focuses visible light rays Can be used on live or dead specimens Dyes and fluorescent markers help for viewing Magnify up to 1000X on the ones we have Examples: Compound light microscope Confocal microscope

Light Microscope

Light Micrographs Plant magnified Human chromosomes 

Confocal Microscope

Confocal micrograph Spirogyra crassa Maximum projection green: Actin. Rhodamin, Label: Phalloidin. Actin red: Chloroplasts. Autofluorescence Image acquired with the Leica TCS SP2

Electron Microscopy (EM) Focus beam of electrons on the specimen Specimens must be dead Kinds Transmission Electron Microscope (TEM) Cross-section of the material Most detailed of internal of cell Scanning Electron Microscope (SEM) Surface of the specimen Shows texture, but less detail

TEM

TEM

SEM

SEM Ant Pollen Grains

Red Blood Cells SEM Compound Light TEM

Compare cardiac myocytes Confocal microscope Compound light microscope neonatal cardiac myocytes from rat green: Myomesin, red: Cadherin blue: DNA (nuclei),

Keen eyes Light microscopes- will not show you something the size of a virus! Do not display in great detail. Confocal microscopy uses brightly colored markers TEM is detailed, flat, and black and white SEM is 3D, textured, and black and white