Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells Anthony G. Passerini, PhDa,

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Shear stress magnitude and directionality modulate growth factor gene expression in preconditioned vascular endothelial cells Anthony G. Passerini, PhDa, Amy Milsted, PhDb, Stanley E. Rittgers, PhDa Journal of Vascular Surgery Volume 37, Issue 1, Pages 182-190 (January 2003) DOI: 10.1067/mva.2003.66 Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 1 Schematic shows shear stress protocol applied to HUVEC monolayers. On reaching confluency in static culture, cells were preconditioned at normalized arterial WSS of 15 dynes/cm2for 24 hours to reset baseline gene expression. They then were exposed to various step changes in WSS for 6 hours. Journal of Vascular Surgery 2003 37, 182-190DOI: (10.1067/mva.2003.66) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 2 Mean ± standard deviation expression levels for endothelial genes (n = 3 per gene per shear level). A, END1; B, NOS3; C, PDGFA; D, PDGFB; E, FGF1; and F, FGF2. *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. Expression levels in static cultured cells are shown for comparison only. Journal of Vascular Surgery 2003 37, 182-190DOI: (10.1067/mva.2003.66) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 3 Mean ± standard deviation expression levels for endothelial genes (n = 3 per gene per shear level). A, TGFA; B, TGFB1; C, VEGF; D, IGF1; E, EGF; and F, ACE. *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. Expression levels in static cultured cells are shown for comparison only. Journal of Vascular Surgery 2003 37, 182-190DOI: (10.1067/mva.2003.66) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 4 Mean ± standard deviation END1/NOS3 mRNA expression ratio (n = 3 per shear level). *P <.05 from baseline (15 dynes/cm2) in preconditioned cells. END1/NOS3 expression ratio in static cultured cells is shown for comparison only. Journal of Vascular Surgery 2003 37, 182-190DOI: (10.1067/mva.2003.66) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions