From: Adenosine Receptor Adora2b Plays a Mechanistic Role in the Protective Effect of the Volatile Anesthetic Sevoflurane during Liver Ischemia/Reperfusion.

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From: Adenosine Receptor Adora2b Plays a Mechanistic Role in the Protective Effect of the Volatile Anesthetic Sevoflurane during Liver Ischemia/Reperfusion Anesthes. 2016;125(3):547-560. doi:10.1097/ALN.0000000000001234 Figure Legend: Sevoflurane induces vasodilator-stimulated phosphoprotein (VASP) phosphorylation in platelets and dampens platelet adenosine triphosphate (ATP) release. Platelet-rich plasma (PRP) was incubated with sevoflurane (5% v/v) and then processed for analysis. (A) Platelets were identified in PRP using flow cytometry according to their typical forward scatter/sideward scatter (FSC/SSC) properties. (B) VASP phosphorylation in platelets was measured using an anti–VASP157-fluorescein isothiocyanate (FITC)–labeled antibody. (C) Sevoflurane induces platelet VASP-P157 (n = 3). (D) Sevoflurane decreases platelet ATP release in PRP (n = 5). Antibody fluorescence is given in arbitrary units (a.u.). Data are shown as scatter plots together with mean and SD imposed on the scatter in (C) and as mean and SD in (D). P values are derived from unpaired t tests; ***P < 0.001. ADP = adenosine diphosphate; PGE = prostaglandin E1. Date of download: 11/8/2017 Copyright © 2017 American Society of Anesthesiologists. All rights reserved.

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