Coral Bleaching Experiment

Slides:



Advertisements
Similar presentations
Estimation of Cholesterol Lieberman-Burchard Reaction
Advertisements

EPPING, LQB381 Flow Diagrams Dr Ron Epping LQB381 Unit Coordinator
Experiment 6 Determination Of Cholesterol In HDL
Scientific Method Unit 2. We will set up the scientific method experiment today and will work on it for the next 2 weeks. Mealworm or Tenebrio molitor.
Volume Part I.
(glass pipette, centrifuge, spectrophotometry)
Goals Consider implications of plants being ‘green’ Use O2 creation as a measurement of photosynthesis Test a common claim from textbooks.
Electromagnetic radiation travels through space in the form of a wave, with the distance between two crests of a wave termed a wavelength. At one end.
Photosynthesis & e - transport Pathway thinking How?Why?
Solution Concentration. Concentration Describes the amount of solute dissolved in a specific amount of solvent.
Sit with last week’s group. Turn in… Dilutions worksheet.
 Line graph - Measures change over time  Measures counted information.
Determination of urine glucose using O-Toluidine 376 BCH Miss Tahani Al-Shehri.
Topic 1 Tools of the Biology Laboratory Exercise I. The spectrophotometer. Exercise II. Liquid Measurements. Exercise III. Sedimentation and Centrifugation.
Manual Extraction of DNA from The Blood. - Blood Sample. - Distilled water. Dionized water. - Ice and Plastic bucket.- Materials.
Manual Extraction of DNA from The Blood. Materials - Blood Sample. - Distilled water. Dionized water. - Ice and Plastic bucket.-
Spectrophotometry Ability of molecules to absorb and transmit light energy is the basis of one of the most widely used procedures for determining the concentration.
Serial Dilutions. What are serial dilutions?  Also called a “dilution series”, serial dilutions are, essentially, dilutions of dilutions.
According to the graph, what particle diameter corresponds to a stream velocity of 10 cm / sec ? About 0.2 cm.
QUANTITATIVE ANALYSIS Determining amount of ions present in samples.
Experiment 33 Colorimetric Determination of Iron CHE1181.
Quantitative Estimation of Pentose
Concentration Calculations Answers. 1. What is the formula for calculating molarity? M = moles solute L of solution.
Absorption Spectrum for Plant Pigments
EXP.2 (Quantitative determination of Amylase activity) Introduction:- The purpose of this experiment is to study the enzyme amylase which is found in saliva.
Protocol: Isolation of proteins Determination of protein concentration
How much protein is really in my… -milk? -soy “milk”?
The Effect of Motor Oil After Two Weeks of Exposure
Colorimetric Determination of Iron
Spectrophotometric Determination [Co2+]
Cell Counts by Hemocytometer
Effect of Gasoline on Coral Bleaching
Group: 1 Presentation 2 By: Sammiejo Fat
Motor Oil Coral Bleaching
Vernier Spectrophotometer
Group1 Kathleen Sherlley Sinthia Kasey Sammiejo
Coral Bleaching By Nathalie Regalado Saskia Medina Cristina Lopez
Lab Activity 5 Biuret Method
Coral Bleaching Lab Size Experiment
Coral Reef Bleaching Experiment
Plasma Clearance.
Presentation 2: Group 3 Expiriment
Salinity and Coral Bleaching
PREPARING SOLUTIONS AND REAGENTS
Determination of Mn in Steel (Standard Addition)
An Investigation of Oil on Coral Beaching
Solutions Section 3: Dilutions Dilutions Calculations print 1-3,5-7.
The effects of Gasoline on Aiptasia
Chlorophyll a Measurement
Lab Activity 6 Biuret Method
A graphing calculator is required for some problems or parts of problems 2000.
Coral Bleaching Experiment
BIL 151 HHMI Coral Bleaching Lab
Mass, Volume, & Density.
Group 3 Merleen Emmanuel
Protocol: Isolation of proteins Determination of protein concentration
Coral Bleaching Presentation 4
Ion Exchange worksheet
Lab Activity 7 IUG, Fall 2017 TMZ.
Presented by: Randy McCroskey Lauren, Selima, Kelli, Lacey
DETERMINATION OF GLUCOSE IN BLOOD STREAM
Lab Activity 7 IUG, Fall 2017 TMZ.
Lab Session 7 IUG, 2012 TMZ.
Determination of Mn in Steel (Standard Addition)
Unit 1 Notes: Graphing & Density
A Scientific Investigation by
What amount of heat is required to increase the temperature of 75
Meredith Barnard and Group 4
A Scientific Investigation by
Percentage Yields.
Presentation transcript:

Coral Bleaching Experiment Marcel Alcide Yao Djilan Leonard Desir Lauren Campbell

Procedure 1) Determine the Zoox density 2) Chlorophyll extraction & measurement 3) Determine protein concentration.

1) Determination of the zoox density Weighed 4 polyps 7.7 x 10-3g insertion of polyps into tube containing 2mL sea water. Homogenize for 20 sec. Measure the total homogenate volume/ 4.3 x 108 zoox/g

2) Chlorophyll extraction & measurement Addition of 9mL of acetone to 1mL of homogenate into new tube Vortex for 10 mn Centrifuge for 5mn Decantation Calculation of the amount of chlorophyll

Calculation of the amount of chlorophyll Wavelength 665nm → 647nm → 630nm → Absorbance 0.03 0.015 0.019

Ca = (11.85A665)-(1.54A647)-(0.08A630) 0.33088

Determination of the protein concentration .1mL of .25 mg/mL BSA + 5mL Bradford .1mL of .50mg/mL BSA + 5mL Bradford .1mL of .75 mg/mL BSA + 5mL Bradford .1mL of 1.00 mg/mL BSA + 5mL Bradford .1mL of 1.25 mg/mL BSA + 5mL Bradford .1 ml of homogenate+ 5mL of Bradford Abs(avg) .385 .641 .900 1.116 1.284 .074 Wavelength used: 595nm

Graph

Result .074 = 1.0139x + .0873 X = -.013

Discussion Why did we get a negative result? The use of an insufficient amount of polyps could cause that to happen because the machine used to measure the absorbance is very sensitive. In the next experiment we will use a greater amount of polyps to see if we will get a positive result.

The End