Clinical Practice Microbiology Prof. Dalia Mohsen Prof. in Microbiology CLS 417

E. mail: Dr.dalia@inaya.edu.sa Welcome to CLS 417 Prof. Dalia M. Mohsen Prof. in Microbiology E. mail: Dr.dalia@inaya.edu.sa Office:- 2B-111

OBJECTIVES Students should know the specimens received in the bacteriology laboratory, their collection techniques, suitability, and processing. Introduction to the safety measures implemented in the laboratories. The culture media preparation and sterilization. processing of specimens, isolation/ identification of organisms, and antibiotic susceptibility testing. The diagnostic techniques in the routine Clinical Immunology, Mycology and Virology laboratories will also be taught.

What this?

OVERVIEW Microbiology:- Microbiology can be defined as the biology of microscopic organisms, or life too small to be seen with the naked eye. Microbiology covers several disciplines, including virology (study of viruses), bacteriology (study of bacteria), mycology (study of fungi), and parasitology (study of parasites).

Clinical microbiology:-  Is a branch that concerned with the prevention, diagnosis and treatment of infectious diseases. In addition, this field of science studies various clinical applications of microbes for the improvement of health.

Microbiology scientist Antonie van Leeuwenhoek The "Father of Microbiology, born in Delft, Dutch Republic considered to be the first microbiologist. He is best known for his work on the improvement of the microscope first to observe and describe single-celled organisms

Antonie van Leeuwenhoek

Louis Pasteur:- "French chemist was the founder of microbiological sciences. discoveries of the principles of vaccination, microbial fermentation and pasteurization. Robert Koch:-  German physician The founder of modern bacteriology, he is known for his role in identifying the specific causative agents of tuberculosis, cholera, and anthrax and for giving experimental support for the concept of infectious disease.

Louis Pasteur

Robert Koch  

Marks’ Distribution First assessment 10 marks Second assessment quizzes projects Final (practical) exam 20 marks Final (theory) exam 40 marks Total 100marks

Topics to be covered

General bench specimens Bacteriological culture media Urine specimen Blood culture Stool specimen General bench specimens Serological test Review in diagnosis of viral infection Review in diagnosis of fungal infection

Required Textbook Cossart, P., P. Boquet, S. Normark, and R. Rappuolo. Cellular Microbiology. ASM Press, Washington, DC, 2000 Vandepitte ,J and Verhaegen J,Engbaek K,Rohner P,Poit P,and C C. Heuck. .Basic laboratory in Clinical Bacteriology.WHO,Geneva,2nd Edition 2003 Kaufmann, S. H. E., A. Sher and R. Ahmed. Immunology of Infectious Diseases. ASM Press, Washington, DC, 2001 Madigan, M. T., Martinko, J. M., and J. Parker. Brock Biology of Microorganisms. Prentice Hall, Upper Saddle River, NJ, 1997 Mims, C., A. Nash, and J. Stephen. Mims' Pathogenesis of Infectious Disease. Academic Press, San Diego, CA. 2001