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From: Lipid-Modifying Enzymes in Human Tear Fluid and Corneal Epithelial Stress Response Invest. Ophthalmol. Vis. Sci.. 2014;55(1):16-24. doi:10.1167/iovs.13-12577 Figure Legend: Stressed HCE cells release phospholipases in the medium. (A) The ASM and NSM2 present in media from UV-B-stimulated cells. (B) The SMase secretion induced by hyperosmolarity stress in HCE cells (50–140 mM of NaCl were added to the growth medium to increase osmolarity). (C) The SMase secretion in LPS-stimulated cells. Cells were stimulated with 70 to 140 mM added concentrations of a NaCl/KCl solution to produce hyperosmolar conditions, UV-B with radiation energy within 1 to 25 mJ/cm2 in a UV crosslinker (Spectroline XL-1500; Spectroline, Westbury, NY), and LPS (E. coli O55:B5; Sigma-Aldrich, St. Louis, MO) in concentrations ranging from 1 ng to 1 lg of LPS/mL medium. Media (200 lL) from control and stressed cells were loaded per lane on a 4% to 12% Bis-Tris NuPAGE gel. LMW, low molecular weight marker. Date of download: 11/9/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.