Genomic and cDNA Libraries Chapter 3 Genomic and cDNA Libraries © 2012 John Wiley & Sons, Ltd.

Figure 3.1 Making a genomic library.

Figure 3.2 Genomic libraries: overlapping and non-overlapping fragments.

Figure 3.3 Using a partial digest to produce a collection of overlapping fragments.

Figure 3.4 Amplification of gene libraries.

Figure 3.5 Assessing the quality of a gene library.

Figure 3.6 Principle of oligo(dT) purification of mRNA.

Figure 3.7 Purification of mRNA through an oligo(dT) column.

Figure 3.8 Synthesis of cDNA from mRNA.

Figure 3.9 cDNA synthesis: using homopolymer tailing.

Figure 3.10 Cloning cDNA.

Figure 3. 11 cDNA synthesis: enhancing representation of 5 mRNA ends Figure 3.11 cDNA synthesis: enhancing representation of 5 mRNA ends. RT, reverse transcriptase.

Figure 3.12 Melting (denaturation) of DNA.

Figure 3.13 Strength of association of base pairs.

Figure 3.14 Formation of hybrid DNA between similar but non-identical DNA molecules.

Figure 3.15 High and low stringency washing.

Figure 3.16 Screening a gene library by hybridization with a gene probe.

Figure 3.17 Southern blotting.