MS media Murashige and Skoog.

Slides:

Advertisements

Similar presentations

Experiment No 2. 5 Experiment Material and Chemicals Overview Introduction Procedure Objective

Advertisements

ASEPTIC TECHNIQUE. PERSONAL PROTECTIVE EQUIPMENT includes: Eye protection Gloves Masks Gowns Sterile technique is employed to prevent contamination of.

Aseptic Technique: Media and Equipment

Introduction Study of cells, tissues or organs in vitro More than 24 hours Behaviour of animal cell without variations in the animal.

Horticultural Science Horticulture CD

Lab procedures when handling micro-organisms Distribution of micro-organisms in nature.

Plant Tissue Culture.

Plant Tissue Culture Do you want a footer?.

How To Prepare, Sterilize, AND Test Culture Media

Media Prep. & Model Organisms Lab 4E, 4F & 2B. Timeline/Overview Monday: Lecture Tuesday: Media Prep (4E & 4F) Pour Plates for Day 2 of Lab 2B Thursday:

Sterilization. Sterile Technique  Important to keep work area as clean as possible  Prevents introduction of other microorganisms from the environment.

Microbiology Safety w Microbes are present everywhere. w Harmful microbes should not be allowed to grow and develop w PRECAUTIONS w All surfaces should.

L8 – Keeping things sterile

Experiment No 1. 5 Experiment Material and Chemicals Overview Introduction Procedure Objective

PHT351 Sterile Dosage Form.

Culture Media Lab 2:.

Biotechnology and Safety

Biotechnology basics State that biotechnology is the industrial use of living organisms (or parts of them) to produce food, drugs or other products. What.

Disinfection and Sterilization.

Structure, function and growth of prokaryote and eukaryote cells (iii) Differentiation of cells into tissues and organs. (iv) Cell and tissue culture –

Mass multiplication of biofertilizers

Aseptic Technique: Media and Equipment. Growth Medium A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms.

Horticulture Science Lesson 19 Propagating Plants by Tissue Culture

TOPIC :- EVALUATION OF ANTIMICROBIAL EFFECTIVENESS & FUNCTION OF LAF SUBMITTED BY :- GAURAV KUMAR PAL M.Sc.Applied Microbiology.

Lab 2: Culture Media. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any.

Isolation technique with aseptic techniques to cultivate bacteria

Plant Tissue Culture.

Source of M.O in Foods.

Preparation Of Cauliflower Explants 1.) Rinse cauliflower head with water. Break or cut cauliflower head into pieces 1 cm long and 3-4 mm in diameter.

Sterile Products Lab PHT 434

Plant and Mammalian Tissue Culture Culture Systems and Aseptic Technique.

INTRODUCTION The procedure involves isolation, mass culture production and preparation of inoculants along with inoculant quality control. The individual.

Plant Tissue Culture Project. What is Plant Tissue Culture? Plant tissue culture is a form of asexual propagation of plants under laboratory conditions.

Basic Principles & Protocol in Plant Tissue Culture

Propagating Plants by Tissue Culture

Key Area 3: Producing New Cells. Mitosis Why do cells divide? Organisms would only ever exist as single cells – fine for bacteria but not so good for.

Essential Laboratory Equipment for Cell Culture Dr. Khalid Enan DFG-Sponsored Workshop, Assuit University, Egypt 10 Sep 2012.

1. 2 Microbiology is the science that deals with the study of too small organism (micro-organisms) that are invisible to the naked eye.

Controlling of Microbial Growth

What is Tissue Culture A very technical method of asexual propagation The growing of plantlets from small pieces of plant tissue from a parent plant.

Distribution System If microorganism colonize a storage vessel, the latter then acts as a microbial reservoir and contaminates all water passing through.

Naomi Asomani Antwi Matilda Ntowah Bissah May, 2016.

Sterilization Lab 3 Abeer Saati.

Working safely with Biological materials Aseptic technique, sterilization and tissue culture techniques.

Laboratory Arrangements, Aseptic Technique, Media, Plant Growth Hormones Jebunnesa Chowdhury.

National 5 Biology Course Notes Unit 1 : Cell Biology Part 3 : Producing new cells.

ASEPTIC TECHNIQUE.  Laboratory practices that minimize the risk of cultured cells becoming contaminated with unwanted microorganisms.

Basic Microbiology and Immunology Practical Course 2016.

Aeration 1-If the plant part is grown on the surface of a semi solid medium it acquires sufficient aeration without a special device. 2-But if liquid medium.

Hannah Jernigan University of Wyoming

TCC techniques-1: Basic Aseptic Rules

Inoculation Incubation Isolation Inspection Identification 5/6/2018

Lab procedures when handling micro-organisms

Aim- Surface sterilization of plant material

Aseptic Technique Definitions.

Marine Biotechnology Lab

Laboratory facilities

Learning Objectives i Understand the basic aseptic techniques used in culturing organisms. ii Understand the principles and techniques involved in culturing.

PATHOLOGICAL TOOLS AND TECHNIQUES. Clearing & preparation of glassware  Glassware are boiled in hot water before washing.  Sock the glassware in soap.

National 5 Biology Producing New Cells.

MATERIALS: Murashige & Skoog Medium (MS) Measuring cylinders

Plant Tissue Culture Do you want a footer?.

Culture Media Lab 2:.

Lab procedures when handling micro-organisms

& presents Plant Tissue Culture.

INTRODUCTION The procedure involves isolation, mass culture production and preparation of inoculants along with inoculant quality control. The individual.

Aseptic Technique & Streaking for Isolation

Plant Tissue Culture Definitions and How To’s

Plant Tissue Culture?.

Presentation transcript:

MS media Murashige and Skoog

aseptic condition 1-Utensils, glass ware, nutrient medium, culture room, inoculation chamber must be in aseptic condition. 2-microbes compete with growing tissue and finally kill it 3-maintain aseptic condition in and around the culture equipments in tissue culture experiments.

*There are three sources of contamination: (i) through' utensils and culture medium (ii) through explant material (iii) through inoculation (transfer of explant to culture medium)

*avoid contamination through utensils - surface sterilized with -sodium hypochlorite (bleaching powder) or -calcium hypochlorite or -chlorine water or -HgCl2 (0.1 %) and *autoclaved at 121°C for 30 minutes for complete sterilization. *Microorganisms get killed at this temperature by autoclaving. *Explant is thoroughly washed with chlorine water or calcium hypochlorite and then with distilled water.

*To prevent the entry of microbes at the time of inoculation, laminar air flow cabinet (chamber used for culture) is sterilized with ultra violet light or filter sterilized air. *The principal advantage of working in these cabinets is that the flow of air does not hamper the use of spirit lamp. *Under tropical and subtropical areas, where dust particles are very high, it must be kept in double door fitted culture room in order to prolong the effective life of the filter.