Affect of DNA-PK on Foci Persistence J. Dickey

J Cell Controls (0 Gy) 0 hr 48 hr 24 hr 72 hr

K Cell Controls (0 Gy) 0 hr 48 hr 24 hr 72 hr

J Cells 3 Gy 0.5 hr 1 hr 3 hr 6 hr 10 hr 24 hr 48 hr 72 hr

K Cells 3 Gy 0.5 hr 1 hr 3 hr 6 hr 10 hr 24 hr 48 hr 72 hr

J Cells 5 Gy 0.5 hr 1 hr 3 hr 6 hr 10 hr 24 hr 48 hr 72 hr

K Cells 5 Gy 0.5 hr 1 hr 3 hr 6 hr 10 hr 24 hr 48 hr 72 hr

J vs. K cells 10 hr post 3 Gy IR J Cells K Cells

J vs. K cells 10 hr post 5 Gy IR J Cells K Cells

Conclusions J cells (deficient in DNA-PK expression) have greater foci persistence over time after IR. Overall numbers of foci between J cells and K cells at 30 min post-IR seem similar. DNA-PK seems to play a role in foci removal.