From: A Hierarchy of Proliferative Cells Exists in Mouse Lens Epithelium: Implications for Lens Maintenance Invest. Ophthalmol. Vis. Sci.. 2006;47(7):2997-3003. doi:10.1167/iovs.06-0130 Figure Legend: Wounding stimulates the central lens epithelial cells to proliferate. Scrape wounds were created on the lenses of mice, labeled in an in utero/postnatal protocol, and chased for 18.5 weeks. Twenty-four hours after wounding, BrdU was then injected intraperitoneally to tag proliferating cells. These mice were killed 24 hours later (A) and paraffin-embedded sections were processed for BrdU immunohistochemistry and autoradiography. Arrowheads: wound area. Note the increased number of BrdU labeled cells in corneal (c) and lens (l) epithelia. A portion of the central zone of the lens epithelium (area in the rectangle) containing BrdU labeled cells (arrow) is shown in higher magnification (B). (C) Paraffin-embedded section of a portion of the central zone of the lens epithelium from another mouse 24 hours post-wounding showing numerous flattened lens epithelial cells stained with BrdU (arrows). (D) A central lens epithelial cell was double-labeled, containing both 3H-TdR and BrdU. This indicates that the LRCs (lrc) were healthy and can divide after wounding. (E) A portion of a nonwounded, resting cornea (c) and lens (l) that were labeled with a single intraperitoneal injection of BrdU and killed 24 hours later. A portion of the central zone of the lens epithelium (area in the rectangle) containing a single BrdU-labeled cell (arrow) is shown in higher magnification (F). Date of download: 11/9/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.