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From: Role of Nectin-1, HVEM, and PILR-α in HSV-2 Entry into Human Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci.. 2009;50(6):2878-2887. doi:10.1167/iovs.08-2981 Figure Legend: HSV-2 is capable of productively replicating in RPE cells. (A) Confluent monolayers of Vero, RPE, and B78H1 cells were infected with wild-type HSV-2(333) at 0.01 PFU/cell for 2 hours at 37°C. Cells were fixed at 24, 48, and 72 hours after infection and stained with Giemsa. Plaques were counted, and data represented the mean ± SD of results performed in triplicate in a representative experiment. (B) Typical Giemsa-stained plaques in Vero, RPE, and B78H1 cells 48 hours after infection with HSV-2(333) are shown. Microscopy was performed with the 10× microscope objective. Advanced software (SlideBook version 3.0, Olympus) was used for images. Date of download: 11/9/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.