Movie 1 Top and side view volume renderings of filopodia in a HeLa cell expressing mEmerald-Lifeact.The high speed of the dithered mode of lattice light-sheet.

Slides:

Advertisements

Similar presentations

Transmission Electron Microscopy (TEM)

Advertisements

What was the state of the technology before the development reported in this paper? What problems does the new development solve? Intro Questions for Technique.

Super-resolution-nanoscopy Resolvable volumes obtained with current commercial super-resolution microscopes. Schermelleh L et al. JCB 2010;190:

How to Multiply using Lattice. Step 1: How many boxes to you need? 23 X 5 You need 2 boxes on top and 1 on the side.

Comment on “Chromosomal Instability and Tumors Promoted by DNA Hypomethylation” and “Induction of Tumors in Mice by Genomic Hypomethylation” by Allen S.

Fig. 1. Structure and optical properties of bR. S Schenkl et al. Science 2005;309: Published by AAAS.

Arecibo Radar Mapping of the Lunar Poles: A Search for Ice Deposits by N. J. S. Stacy, D. B. Campbell, and P. G. Ford Science Volume 276(5318):

Computational Experiments for Science Education by Charles Xie, Robert Tinker, Barbara Tinker, Amy Pallant, Daniel Damelin, and Boris Berenfeld Science.

Endosomal Proteolysis of the Ebola Virus Glycoprotein Is Necessary for Infection by Kartik Chandran, Nancy J. Sullivan, Ute Felbor, Sean P. Whelan, and.

Current Problems in the Management of Marine Fisheries by J. R. Beddington, D. J. Agnew, and C. W. Clark Science Volume 316(5832): June 22, 2007.

Open Source Physics by Wolfgang Christian, Francisco Esquembre, and Lyle Barbato Science Volume 334(6059): November 25, 2011 Published by AAAS.

Fig. 1 Methods of light-sheet microscopy

Movie 7 Rapid movements of a single-cell protozoan T

Requirement of DCC, but not neogenin, expression for the basal distribution of GFP–Myo X. (A) 3D confocal imaging analysis of NLT cells expressing GFP–Myo.

by Jennifer Couzin-Frankel

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Cities are the Future by Nicholas S. Wigginton, Julia Fahrenkamp-Uppenbrink, Brad Wible, and David Malakoff Science Volume 352(6288): May 20, 2016.

Rethinking the global supply chain

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

FUS Zigzags Its Way to Cross Beta

by Douglas D. Ross, Judith E. Karp, Tar T. Chen, and L. Austin Doyle

Torus-Margo Pits Help Conifers Compete with Angiosperms

Microscopy, Meet Big Data

Your Lecture you only have 8 minutes MAX 8 slides Start off with title Then give a 1 or 2 sentence introduction and conclusion Give me a topic/research.

Social-Ecological Resilience to Coastal Disasters

Comment on “Impacts of species richness on productivity in a large-scale subtropical forest experiment” by Hua Yang, Zhongling Guo, Xiuli Chu, Rongzhou.

Surface area and volume: Surface area of a cuboid

Submitochondrial localization of mitofilin using quantitative immunoelectron microscopy. Submitochondrial localization of mitofilin using quantitative.

Fig. 1 Culturomic analyses study millions of books at once.

by Lidong Qin, Sungho Park, Ling Huang, and Chad A. Mirkin

A Microfluidic Device for Conducting Gas-Liquid-Solid Hydrogenation Reactions by Juta Kobayashi, Yuichiro Mori, Kuniaki Okamoto, Ryo Akiyama, Masaharu.

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics by Dong Li, Lin Shao, Bi-Chang Chen, Xi Zhang, Mingshu Zhang,

by Jaakko V. I. Timonen, Mika Latikka, Ludwik Leibler, Robin H. A

Mitofilin is localized in individual clusters in the mitochondria of primary adult human fibroblasts. Mitofilin is localized in individual clusters in.

Student-Directed Discovery of the Plant Microbiome and Its Products

Fig. 2 In vitro assessment of hESC-RPE cell sheets.

The Antisense Transcriptomes of Human Cells

by William T. S. Cole, James D. Farrell, David J. Wales, and Richard J

STED microscopy of MINOS

Cover stories: Making the robotic ray cover

Emerging Imaging and Genomic Tools for Developmental Systems Biology

Cytokinesis: Going Super-Resolution in Live Cells

by Jungwon Park, Hans Elmlund, Peter Ercius, Jong Min Yuk, David T

How to Multiply using Lattice

by Jelena Stajic, Richard Stone, Gilbert Chin, and Brad Wible

Fig. 2 Analysis of CLL lymph nodes.

Congratulations! Now Get to Work

Endolysosomal proteins are exported from the Golgi complex in two distinct populations of transport carriers. Endolysosomal proteins are exported from.

Segregation of endolysosomal proteins in the Golgi complex.

Fig. 1 Raw data from Ota et al

Fig. 2. An automated cell phone–based video microscope.

by Christopher Battle, Chase P. Broedersz, Nikta Fakhri, Veikko F

HJURP is required for CENP-A localization to centromeres.

A Drosophila cell culture model to study VAP(P58S) aggregation.

Fig. 1. E-cadherin localizes in nano-scale clusters.

by Fei Chen, Paul W. Tillberg, and Edward S. Boyden

Two approaches for improved live-cell imaging at sub-100-nm resolution

SBF-SEM of mitotic spindles.

The SIM domain of RAD51 is required for HR

Single MTs can be resolved in subsections of SBF-SEM image stacks.

LC8 and its binding partners display broad cellular localization.

Peroxisome speeds were slower in patient and control cells.

Fig. 1. Expression of a Ror-eGFP fusion protein under control of the endogenous Ror promoter in Drosophila embryos. Expression of a Ror-eGFP fusion protein.

Ub-binding ability of ERα.

Social support receipt stratified by recipient’s age and gender and by provider’s gender and relationship to recipient. Social support receipt stratified.

2DG suppresses of lamellipodia and filopodia and causes disorganization of F-actin filaments in murine endothelial cells. 2DG suppresses of lamellipodia.

Nuclear invaginations observed in thin sections are 3D tunnels and crevices, as shown by FIB-SEM and STORM. (A) In thin section TEM, large nuclear invaginations.

Effect of SPINDLIN1 protein on cancer cell proliferation and invasion.

Illustration of the point-counting technique applied to estimate hippocampal volume from MR images of a control (C, top row), patient with left-sided seizure.

Presentation transcript:

Movie 1 Top and side view volume renderings of filopodia in a HeLa cell expressing mEmerald-Lifeact.The high speed of the dithered mode of lattice light-sheet microscopy (left) is compared against the high resolution of the SR-SIM mode (right). Top and side view volume renderings of filopodia in a HeLa cell expressing mEmerald-Lifeact.The high speed of the dithered mode of lattice light-sheet microscopy (left) is compared against the high resolution of the SR-SIM mode (right). Play video Bi-Chang Chen et al. Science 2014;346:1257998 Published by AAAS