From: Induction of Functional 3D Ciliary Epithelium–Like Structure From Mouse Induced Pluripotent Stem Cells Invest. Ophthalmol. Vis. Sci.. 2016;57(1):153-161.

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From: Induction of Functional 3D Ciliary Epithelium–Like Structure From Mouse Induced Pluripotent Stem Cells Invest. Ophthalmol. Vis. Sci.. 2016;57(1):153-161. doi:10.1167/iovs.15-17610 Figure Legend: Expression of ciliary marker on E16.5 and P5 mouse eye, and 3D culture of mouse iPS and ES cell aggregates. (A–E) Immunohistochemistry for ciliary marker in E16.5 (A, B) and P5 (C–E) C57BL/6J mouse eye. (A) Cx43-positive staining is observed in the lens epithelium, CE (NPE and PE), gap junctions between PE (arrows) and NPE (arrowheads), and RPE. (B) AQP1-positive staining is observed in the NPE and cornea. (C) AQP1-positive staining is observed in the NPE and iris, especially in the anterior pars plicata. (D) AQP4-positive staining is observed in the NPE, especially in the posterior pars plicata. (E) Cx43-positive staining is observed in the CE (NPE and PE), gap junction, RPE, and posterior region of the iris. The NPE and PE are opposed to each other. Scale bars: 50 μm (F–I) Optic cup–like structure in 3D culture (Eiraku's protocol) from mouse ES/iPS cells. Optic cup–like structure on day 11 (F) and day 13 (G) from mouse iPS cells and day 10 (H, I) from mouse ES cells. (I) Optic cup–like structures were positive for Rx-GFP. The NPE-like cells (arrowheads) and PE/RPE-like cells (arrows) are continuous and not opposed to each other. Scale bars: 200 μm. (J–M) Immunohistochemistry of cryosections of optic cup–like aggregates from mouse iPS cells. (J) DAPI-stained nuclei reveal the morphology of optic cup-like aggregates. (K) Cx43-positive staining was only observed in the outer layer, and this marker was not expressed at all in the inner layer of the optic cup–like aggregate. (L, M) AQP1-positive staining was also observed in the outer layer, and similar to Cx43, this marker was not expressed at all in the inner layer of the optic cup–like aggregate. The NPE-like cells (arrowheads) and PE/RPE-like cells (arrows) are located next to each other. Scale bars: 50 μm. Date of download: 11/9/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.