Half Adder - Modeling Overview

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Presentation transcript:

Half Adder - Modeling Overview September 4th, 2006 ETH Zürich | iGEM team 2006

System Architecture Half-Adder Light Sensing PoPS Duplexer Light PoPS to RFP XOR PoPS PoPS to GFP AND Chemical Sensing PoPS Duplexer PoPS PoPS PoPS

Insights Overview

Light Sensing ho1 PcyA Cph1 Cph1 Cph1 EnvZ EnvZ EnvZ P OmpR P OmpR Heme PCB ho1 PcyA PCB PCB Cph1 Cph1 Cph1 EnvZ EnvZ EnvZ P Auto-Phosphorylation OmpR P Part: BBa_I15010 OmpR Cph8: Fusion Cph1/EnvZ Chimeric Cph1 light receptor/EnvZ protein Part:BBa_I15008 Heme Oxygenase (ho1) PoPS Part:BBa_I15009 Phycocyanobilin (PcyA) ferredoxin oxidoreductase Part: BBa_R0082

Chemical Sensing LacI LacI IPTG IPTG IPTG PoPS Part: BBa_Q01121 BBa_R0011 Strong promoter (lacI regulated) BBa_B0012 Terminator BBa_I14032 Strong constitutive ON promoter BBa_B0031 RBS BBa_C0012 LacI repressor BBa_B0010 Terminator

PoPS Duplexer D1 D2 D3 D4 Light PoPS Duplexer Chemical PoPS Duplexer

AND (Version A) PoPS e1 E1 PoPS E1 E2 E2 PoPS e2

AND (Version B) PoPS a R A PoPS r1 r2 B PoPS R b

XOR I1 PoPS i1 i2’ I1’ I1 - I1‘ PoPS I2 - I2‘ PoPS I2’ i2 i1’ I2

PoPS to Reporter GFP PoPS gfp RFP PoPS rfp