Nolwazi L. Bhembe, Ishmael F. Jaja, Uchechukwu U. Nwodo, Anthony I

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Prevalence of tuberculous lymphadenitis in slaughtered cattle in Eastern Cape, South Africa Nolwazi L. Bhembe, Ishmael F. Jaja, Uchechukwu U. Nwodo, Anthony I. Okoh, Ezekiel Green International Journal of Infectious Diseases Volume 61, Pages 27-37 (August 2017) DOI: 10.1016/j.ijid.2017.05.005 Copyright © 2017 The Author(s) Terms and Conditions

Figure 1 Map showing the location of Queenstown within the Lukanji Local Municipality and the location of East London within the Buffalo City Metropolitan Municipality. International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions

Figure 2 Detection of MTBC through the amplification of the IS6110 sequence. Lane 1: 100-bp DNA ladder; lane 2: Mycobacterium tuberculosis positive control (H37Rv); lanes 3–13: amplicons of MTBC; lane 14: negative control (nuclease-free water). International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions

Figure 3 Detection of MTBC through the amplification of the mpb64 gene. Lane 1: 100-bp DNA ladder; lane 2: Mycobacterium tuberculosis positive control (H37Rv); lanes 3–12: amplicons of MTBC; lane 13: negative control (nuclease-free water). International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions

Figure 4 Nested PCR detection of MTBC through the amplification of the IS6110 and mpb64 genes. Lane 1: 100-bp DNA ladder; lane 2: Mycobacterium tuberculosis positive control (H37Rv); lane 3: negative control (nuclease-free water); lanes 4–13: amplicons of MTBC. International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions

Figure 5 Characterization of Mycobacterium bovis and Mycobacterium tuberculosis from Mycobacterium bovis BCG through the amplification of RD1 (region of deletion 1). Lane 1: Universal KAPA DNA ladder; lane 2: Mycobacterium tuberculosis positive control (H37Rv); lane 3: negative control (nuclease-free water); lanes 4–12: MTBC detected isolates. DNA amplicons loaded in lanes 8 and 11 were re-amplified to confirm the 511-bp bands. International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions

Figure 6 Amplification of the oxyR gene prior to digestion. Lane 1: 100-bp DNA ladder; lane 2: negative control (nuclease-free water); lane 3: Mycobacterium tuberculosis positive control (H37Rv); lanes 4–8: MTBC isolates. International Journal of Infectious Diseases 2017 61, 27-37DOI: (10.1016/j.ijid.2017.05.005) Copyright © 2017 The Author(s) Terms and Conditions