Date of download: 11/9/2017 Copyright © ASME. All rights reserved. From: Thermally Induced Apoptosis, Necrosis, and Heat Shock Protein Expression in Three-Dimensional Culture J Biomech Eng. 2014;136(7):071006-071006-10. doi:10.1115/1.4027272 Figure Legend: Caspase 3 activity of PC3 cells heated at various temperatures and durations assessed by Ac-DEVD-AMC fluorometric assay. Cells heated for 3 or 5 min (blue markers) and 10 or 22 min (red markers) at 37 °C (+), 40 °C (○), 44 °C (□), 50 °C (♦), 55 °C (*), 56 °C (▾), 57 °C (Δ), or caspase extract positive control (◁). Cells were processed and analyzed at 6, 12, and 24 h after heat shock. Assay duration is approximately 3 h. Y axis units are arbitrary. No caspase 3 activity from PC3 cells was detected.