From: Canonical/β-Catenin Wnt Pathway Activation Improves Retinal Pigmented Epithelium Derivation From Human Embryonic Stem Cells Invest. Ophthalmol. Vis.

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From: Canonical/β-Catenin Wnt Pathway Activation Improves Retinal Pigmented Epithelium Derivation From Human Embryonic Stem Cells Invest. Ophthalmol. Vis. Sci.. 2015;56(2):1002-1013. doi:10.1167/iovs.14-15835 Figure Legend: Fluorescent reporters are RPE-specific and reflect endogenous gene expression. (A) Fluorescent images show that RAX-, OTX2-, MITF-D–, and RPE65-mCherry reporters are expressed in hESC (H9)–RPE and hfRPE, but not in negative control endothelial cells (HUVECs) after 4 weeks in culture. Scale bar represents 100 μm. (B) Quantitative real-time PCR analysis of RPE-specific endogenous gene expression correlates with reporter fluorescence. Error bars represent the standard error of the mean (SEM). Date of download: 11/9/2017 The Association for Research in Vision and Ophthalmology Copyright © 2017. All rights reserved.